Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues

We have developed a DNA extraction procedure for milligram amounts of plant tissue. Yields ranged from 0.3-200 nanograms of DNA per milligram of tissue. The factors affecting yield are discussed. Fresh tissue, as well as herbarium specimens (22-118 years old) and mummified seeds and embryos (500 to...

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Published in	Plant molecular biology Vol. 5; no. 2; pp. 69 - 76
Main Authors	Rogers, S.O, Bendich, A.J
Format	Journal Article
Language	English
Published	Netherlands 01.01.1985
Subjects	DNA extraction methodology plants tissues
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Abstract	We have developed a DNA extraction procedure for milligram amounts of plant tissue. Yields ranged from 0.3-200 nanograms of DNA per milligram of tissue. The factors affecting yield are discussed. Fresh tissue, as well as herbarium specimens (22-118 years old) and mummified seeds and embryos (500 to greater than 44 600 years old) were used. All tissues attempted (57 types from 29 species) yielded measurable amounts of DNA. In no case tested was inhibition observed for restriction enzymes BamHI or EcoRI.
AbstractList	We have developed a DNA extraction procedure for milligram amounts of plant tissue. Yields ranged from 0.3-200 nanograms of DNA per milligram of tissue. The factors affecting yield are discussed. Fresh tissue, as well as herbarium specimens (22-118 years old) and mummified seeds and embryos (500 to greater than 44 600 years old) were used. All tissues attempted (57 types from 29 species) yielded measurable amounts of DNA. In no case tested was inhibition observed for restriction enzymes BamHI or EcoRI.
Author	Rogers, S.O Bendich, A.J
Author_xml	– sequence: 1 fullname: Rogers, S.O – sequence: 2 fullname: Bendich, A.J
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/24306565$$D View this record in MEDLINE/PubMed
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References	4517783 - Nat New Biol. 1973 Oct 10;245(145):170-2 24424833 - Planta. 1976 Jan;131(3):293-8 4558510 - Brookhaven Symp Biol. 1972;23:451-94 334774 - J Biol Chem. 1977 Nov 25;252(22):8118-25 16659109 - Plant Physiol. 1975 Mar;55(3):496-501 6977 - Philos Trans R Soc Lond B Biol Sci. 1976 May 27;274(933):227-74 7433111 - Nucleic Acids Res. 1980 Oct 10;8(19):4321-5 6504142 - Nature. 1984 Nov 15-21;312(5991):282-4
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Snippet	We have developed a DNA extraction procedure for milligram amounts of plant tissue. Yields ranged from 0.3-200 nanograms of DNA per milligram of tissue. The...
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SubjectTerms	DNA extraction methodology plants tissues
Title	Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues
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