Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues

We have developed a DNA extraction procedure for milligram amounts of plant tissue. Yields ranged from 0.3-200 nanograms of DNA per milligram of tissue. The factors affecting yield are discussed. Fresh tissue, as well as herbarium specimens (22-118 years old) and mummified seeds and embryos (500 to...

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Bibliographic Details
Published inPlant molecular biology Vol. 5; no. 2; pp. 69 - 76
Main Authors Rogers, S.O, Bendich, A.J
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.1985
Subjects
DNA
extraction
methodology
plants
tissues
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Summary:We have developed a DNA extraction procedure for milligram amounts of plant tissue. Yields ranged from 0.3-200 nanograms of DNA per milligram of tissue. The factors affecting yield are discussed. Fresh tissue, as well as herbarium specimens (22-118 years old) and mummified seeds and embryos (500 to greater than 44 600 years old) were used. All tissues attempted (57 types from 29 species) yielded measurable amounts of DNA. In no case tested was inhibition observed for restriction enzymes BamHI or EcoRI.
ISSN:0167-4412
1573-5028
DOI:10.1007/bf00020088