Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?

Abstract Purpose To determine by histomorphometric analysis whether CO2 pneumoperitoneum interferes with collagen deposition in surgical wounds in the aponeurosis of rats. Methods This experiment involved 80 male Wistar rats, randomly allocated into four groups according to pneumoperitoneum period (...

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Published inActa cirúrgica brasileira Vol. 35; no. 6
Main Authors Morais, Pedro Henrique Alves de, Silva, Rafael Francisco Alves, Ribeiro, Thiago da Silva, Farias, Igor Eduardo Caetano de, Lino Junior, Ruy de Souza, Carneiro, Fabiana Pirani, Durães, Leonardo de Castro, Sousa, João Batista de
Format Journal Article
LanguageEnglish
Published Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 01.01.2020
Subjects
Collagen
Incisional Hernia
Laparoscopy
Minimally Invasive Surgical Procedures
Pneumoperitoneum
Rats
Wound Healing
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Summary:Abstract Purpose To determine by histomorphometric analysis whether CO2 pneumoperitoneum interferes with collagen deposition in surgical wounds in the aponeurosis of rats. Methods This experiment involved 80 male Wistar rats, randomly allocated into four groups according to pneumoperitoneum period (PRE: 30 min preoperatively; POST: 30 min postoperatively; PP: 30 min pre- and postoperatively; C: control group). CO2 pneumoperitoneum was insufflated to 5 mmHg of pressure. A laparotomy was performed; 1 cm of the left colon was then resected, and an end-to-end anastomosis was performed to simulate surgical trauma, after which the abdominal wall was closed. On postoperative days 7 or 14, a sample of the abdominal wall was collected, stained with picrosirius red and observed under polarized light in an optical microscope. The amount of collagen was estimated by computerized histomorphometric analysis. Results There were no significant differences in collagen deposition between the control and experimental groups on postoperative days 7 (p=0.720) or 14 (p=0.933). The amount of collagen increased as expected in all groups between postoperative days 7 and 14 (p=0.0003). Conclusion At 5 mmHg, CO2 pneumoperitoneum does not interfere with collagen deposition in abdominal wall surgical wounds in rats.
ISSN:0102-8650
1678-2674
DOI:10.1590/s0102-865020200060000005