Change in Tissue Microbiome and Related Human Beta Defensin Levels Induced by Antibiotic Use in Bladder Carcinoma

• Published in: International journal of molecular sciences Vol. 25; no. 8; p. 4562
• Main Authors: Monyók, Ádám, Mansour, Bassel, Vadnay, István, Makra, Nóra, Dunai, Zsuzsanna A, Nemes-Nikodém, Éva, Stercz, Balázs, Szabó, Dóra, Ostorházi, Eszter
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.04.2024
• Subjects: Aged; Analysis; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Antibacterial agents; antibiotic treatment; Antibiotics; BCG; BCG vaccines; Beta lactamases; beta-Defensins - metabolism; beta-Lactams - pharmacology; beta-Lactams - therapeutic use; Bladder cancer; Cancer therapies; Cell growth; Female; Fluoroquinolones - pharmacology; Fluoroquinolones - therapeutic use; Fosfomycin - pharmacology; Fosfomycin - therapeutic use; human beta defensins; Humans; Hyperplasia; Male; Microbiota; Microbiota - drug effects; Middle Aged; Patient compliance; Patients; Prostate; Staphylococcal infections; Success; tissue microbiome; Tumors; Urinary Bladder Neoplasms - drug therapy; Urinary Bladder Neoplasms - metabolism; Urinary Bladder Neoplasms - microbiology; Urine; Hungary; Canada; tissue microbiome; antibiotic treatment; bladder cancer; human beta defensins
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25084562

It is now generally accepted that the success of antitumor therapy can be impaired by concurrent antibiotic therapy, the presence of certain bacteria, and elevated defensin levels around the tumor tissue. The aim of our current investigation was to identify the underlying changes in microbiome and defensin levels in the tumor tissue induced by different antibiotics, as well as the duration of this modification. The microbiome of the tumor tissues was significantly different from that of healthy volunteers. Comparing only the tumor samples, no significant difference was confirmed between the untreated group and the group treated with antibiotics more than 3 months earlier. However, antibiotic treatment within 3 months of analysis resulted in a significantly modified microbiome composition. Irrespective of whether Fosfomycin, Fluoroquinolone or Beta-lactam treatment was used, the abundance of decreased, and abundance increased. Large amounts of the genus were observed in the Fluoroquinolone-treated group. Regardless of the antibiotic treatment, hBD1 expression of the tumor cells consistently doubled. The increase in hBD2 and hBD3 expression was the highest in the Beta-lactam treated group. Apparently, antibiotic treatment within 3 months of sample analysis induced microbiome changes and defensin expression levels, depending on the identity of the applied antibiotic.