Differential Gene Expression by RNA-Seq Analysis of the Primo Vessel in the Rabbit Lymph

• Published in: Journal of acupuncture and meridian studies Vol. 12; no. 1; pp. 11 - 19
• Main Authors: Shin, Jun-Young, Choi, Sang-Heon, Choi, Da-Woon, An, Ye-Jin, Seo, Jae-Hyuk, Choi, Jong-Gu, Rho, Min-Suk, Lee, Sang-Suk
• Format: Journal Article
• Language: English
• Published: Korea (South) Medical Association of Pharmacopuncture Institute 01.02.2019; 사단법인약침학회
• Subjects: 한의학; RNA-Seq analysis; gene expression level; primo connectome; lymph node
• ISSN: 2005-2901; 2093-8152
• DOI: 10.1016/j.jams.2018.10.008

For the connectome of primo vascular system, some long-type primo vessels dyed with Alcian blue injected into inguinal nodes, abdominal node, and axially nodes were visualized, which passed over around the vena cava of the rabbit. The Alcian blue dye revealed primo vessels and colored blue in the rabbit lymph vessels. The length of long-type primo vessels was 18 cm on average, of which diameters were about 20-30 μm, and the lymph vessels had diameters of 100-150 μm. Three different tissues of pure primo vessel, mixed primo + lymph vessel, and only lymph vessel were made to undergo RNA-Seq analysis by next-generation sequencing. We also analyzed differentially expressed genes (DEGs) from the RNA-Seq data, in which 30 genes of the primo vessels, primo + lymph vessels, and lymph vessels were selected for primo marker candidates. From the plot of DEG analysis, 10 genes had remarkably different expression pattern on the Group 1 (primo vessel) vs Group 3 (lymph vessel). With Fragments Per Kilobase of exon per Million the cutoff p-value for each gene was < 0.05. Fragments Per Kilobase of exon per Million of the 10 genes such as IGHM, HLA-DRA, HIST1H41, LPL, CD36, SRGN, DGAT2, SNCG, CD48, and GPD1 for primo vessels compared with those of lymph vessels increased twice or thrice. These results suggest that the selected genes could be used for the specific marker to construct primo connectome of circuit system in the rabbit.