Protective Effects of Arctium lappa L. Roots Against Hydrogen Peroxide-Induced Cell Injury and Potential Mechanisms in SH-SY5Y Cells
Accumulated evidence has shown that excessive reactive oxygen species (ROS) have been implicated in neuronal cell death related with various chronic neurodegenerative disorders. This study was designed to explore neuroprotective effects of ethyl acetate extract of Arctium lappa L. roots (EAL) on hyd...
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Published in | Cellular and molecular neurobiology Vol. 35; no. 3; pp. 335 - 344 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.04.2015
|
Subjects | |
Online Access | Get full text |
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Summary: | Accumulated evidence has shown that excessive reactive oxygen species (ROS) have been implicated in neuronal cell death related with various chronic neurodegenerative disorders. This study was designed to explore neuroprotective effects of ethyl acetate extract of
Arctium lappa
L. roots (EAL) on hydrogen peroxide (H
2
O
2
)-induced cell injury in human SH-SY5Y neuroblastoma cells. The cell viability was significantly decreased after exposure to 200 μM H
2
O
2
, whereas pretreatment with different concentrations of EAL attenuated the H
2
O
2
-induced cytotoxicity. Hoechst 33342 staining indicated that EAL reversed nuclear condensation in H
2
O
2
-treated cells. Meanwhile, TUNEL assay with DAPI staining showed that EAL attenuated apoptosis was induced by H
2
O
2
. Pretreatment with EAL also markedly elevated activities of antioxidant enzyme (GSH-Px and SOD), reduced lipid peroxidation (MDA) production, prevented ROS formation, and the decrease of mitochondrial membrane potential. In addition, EAL showed strong radical scavenging ability in 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assays. Furthermore, EAL inhibited H
2
O
2
-induced apoptosis by increases in the Bcl-2/Bax ratio, decreases in cytochrome
c
release, and attenuation of caspase-3, caspase-9 activities, and expressions. These findings suggest that EAL may be regarded as a potential antioxidant agent and possess potent neuroprotective activity against H
2
O
2
-induced injury. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0272-4340 1573-6830 |
DOI: | 10.1007/s10571-014-0129-7 |