Oxymatrine Lightened the Inflammatory Response of LPS-Induced Mastitis in Mice Through Affecting NF-κB and MAPKs Signaling Pathways

• Published in: Inflammation Vol. 37; no. 6; pp. 2047 - 2055
• Main Authors: Yang, Zhengtao, Yin, Ronglan, Cong, Yunfeng, Yang, Zhanqing, Zhou, Ershun, Wei, Zhengkai, Liu, Zhicheng, Cao, Yongguo, Zhang, Naisheng
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.12.2014
• Subjects: Alkaloids - pharmacology; Alkaloids - therapeutic use; Animals; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Biomedical and Life Sciences; Biomedicine; Dose-Response Relationship, Drug; Female; Immunology; Inflammation - drug therapy; Inflammation - metabolism; Inflammation Mediators - antagonists & inhibitors; Inflammation Mediators - metabolism; Internal Medicine; Lipopolysaccharides - toxicity; Male; MAP Kinase Signaling System - drug effects; MAP Kinase Signaling System - physiology; Mastitis - drug therapy; Mastitis - metabolism; Mastitis - pathology; Mice; Mice, Inbred BALB C; NF-kappa B - antagonists & inhibitors; NF-kappa B - metabolism; Pathology; Pharmacology/Toxicology; Quinolizines - pharmacology; Quinolizines - therapeutic use; Rheumatology; Signal Transduction - drug effects; Signal Transduction - physiology; Sophora; oxymatrine; NF-κB; mastitis; LPS; MAPKs
• ISSN: 0360-3997; 1573-2576
• DOI: 10.1007/s10753-014-9937-7

Mastitis, an inflammatory reaction of the mammary gland, is recognized as one of the most costly diseases in dairy cattle. Oxymatrine, one of the alkaloids extracted from Chinese herb Sophora flavescens Ait , has been reported to have many biological activities, such as anti-inflammatory, anti-virus, and anti-hepatic fibrosis properties. The aim of this study was to investigate the protective effect and the anti-inflammatory mechanism of oxymatrine on lipopolysaccharide (LPS)-induced mastitis in mice. The mouse mastitis was induced by 10 μg of LPS for 24 h. Oxymatrine was intraperitoneally administered with the dose of 30, 60, and 120 mg/kg 1 h before and 12 h after LPS induction. The results showed that oxymatrine significantly attenuated the damage of the mammary gland induced by LPS. Oxymatrine inhibited the phosphorylation of NF-κB p65 and IκB in NF-κB signal pathway and reduced the phosphorylation of p38, ERK, and JNK in mitogen-activated protein kinase (MAPKs) signal pathway. The results showed that oxymatrine had a protective effect on LPS-induced mastitis, and the anti-inflammatory mechanism of oxymatrine was related to the inhibition of NF-κB and MAPKs signal pathways.