FREQUENCY OF MULTIPLE INSEMINATIONS IN FIELD-COLLECTED ANOPHELES GAMBIAE FEMALES REVEALED BY DNA ANALYSIS OF TRANSFERRED SPERM

We investigated the frequencies of single and multiple matings in field-collected female Anopheles gambiae by conducting microsatellite DNA analyses on the sperm contained within their spermatheca. Amplifcation by a polymerase chain reaction (PCR) at four loci allowed the detection of sperm extracts...

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Published inThe American journal of tropical medicine and hygiene Vol. 68; no. 1; pp. 1 - 5
Main Authors TRIPET, FREDERIC, TOURE, YEYA T, DOLO, GUIMOGO, LANZARO, GREGORY C
Format Journal Article
LanguageEnglish
Published Lawrence, KS ASTMH 01.01.2003
Allen Press
Subjects
Animals
Anopheles - physiology
Anopheles gambiae
Biological and medical sciences
Culicidae
DNA, Ribosomal - analysis
Female
Fundamental and applied biological sciences. Psychology
Insect Vectors - physiology
Insemination - genetics
Insemination - physiology
Male
Medically important nuisances and vectors, pests of stored products and materials: population survey and control
Microsatellite Repeats
Sexual Behavior, Animal - physiology
Spermatozoa - chemistry
Spermatozoa - classification
Tropical medicine
Vectors. Intermediate hosts
Mali
Africa
Anopheles gambiae
Semen
Arthropoda
Insecta
DNA
Culicidae
Polyandry
Female
Invertebrata
Fecundation
Diptera
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Summary:We investigated the frequencies of single and multiple matings in field-collected female Anopheles gambiae by conducting microsatellite DNA analyses on the sperm contained within their spermatheca. Amplifcation by a polymerase chain reaction (PCR) at four loci allowed the detection of sperm extracts exhibiting more than two alleles per locus, thereby revealing the occurrence of multiple inseminations. Polyandry was found in six of 239 females examined, or 2.5% of the samples. Previous analyses of the molecular form of the sperm and female extracts using a PCR-based diagnostic procedure showed that two of these multiple inseminations involved cross-mating between two chromosomal/molecular forms of An. gambiae s.s. Thus polyandry occurred within-form in 1.7% of examined females while other multiple inseminations may be linked to processes of reproductive isolation between forms of An. gambiae.
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ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.2003.68.1.0680001