Antimicrobial potential of endophytic fungi from Astragalus chinensis
The purpose of the present study was to discover antimicrobial endophytic fungi from Astragalus chinensis. Three fungal endophytes with antibacterial activity were isolated and determined as Chaetomium sp. HQ-1, Fusarium sp. HQ-7 and Fusarium sp. HQ-9 based on the neighbor-joining phylogenetic tree....
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Published in | 3 Biotech Vol. 9; no. 11; pp. 1 - 9 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Cham
Springer International Publishing
01.11.2019
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | The purpose of the present study was to discover antimicrobial endophytic fungi from
Astragalus chinensis.
Three fungal endophytes with antibacterial activity were isolated and determined as
Chaetomium
sp. HQ-1,
Fusarium
sp. HQ-7 and
Fusarium
sp. HQ-9 based on the neighbor-joining phylogenetic tree.
Chaetomium
sp. HQ-1 showed the best antibiotic potential and was thus selected for large-scale fermentation. Bioactivity-directed separation of ME fermentation of strain HQ-1 led to the discovery of three compounds, which were identified as differanisole A (
1
), 2,6-dichloro-4-propylphenol (
2
) and 4,5-dimethylresorcinol (
3
), from the HR–ESI–MS and NMR data analysis. All three compounds exhibited moderate antibacterial activity against
Listeria monocytogenes
,
Staphylococcus aureus
, and methicillin-resistant
S. aureus
, with MIC values ranging from 16 to 128 μg/mL. Compounds
1
and
3
also displayed promising antifungal activity against
Selerotium rolfsii
with IC
50
values of less than 16 and 32 μg/mL, respectively, which were comparable to that of actidione (8 μg/mL). The findings of the present study suggest that the endophytic fungi from
A. chinensis
have the potential to be used as bactericides and fungicides. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2190-572X 2190-5738 |
DOI: | 10.1007/s13205-019-1948-5 |