Parallel one-pot synthesis and structure–activity relationship study of symmetric formimidoester disulfides as a novel class of potent non-nucleoside HIV-1 reverse transcriptase inhibitors
The molecular duplication of non-nucleoside reverse transcriptase inhibitor (NNRTI) O-(2-phthalimidoethyl)– N-arylthiocarbamates (C-TCs) led to the identification of symmetric formimidoester disulfides (DSs) as a novel class of potent NNRTIs. The lead compound 1 [dimer of the isothiocarbamic form of...
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Published in | Bioorganic & medicinal chemistry Vol. 16; no. 12; pp. 6353 - 6363 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Elsevier Ltd
15.06.2008
Elsevier Science |
Subjects | |
Online Access | Get full text |
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Summary: | The molecular duplication of non-nucleoside reverse transcriptase inhibitor (NNRTI)
O-(2-phthalimidoethyl)–
N-arylthiocarbamates (C-TCs) led to the identification of symmetric formimidoester disulfides (DSs) as a novel class of potent NNRTIs. The lead compound
1 [dimer of the isothiocarbamic form of TC
O-(2-phthalimidoethyl)–
N-phenylthiocarbamate] turned out to prevent the wild-type HIV-1 multiplication in MT-4 cell culture with an EC
50 value of 0.35
μM. In order to perform a structure–activity relationship (SAR) study, we prepared 40 analogues of
1 by an unprecedented one-pot method of solution-phase parallel synthesis. The SAR strategy was focused on the variation of the
N-aryl portion (mono-, di- and trisubstitution of the phenyl ring and its replacement with a 1-naphthyl, cyclopropyl or benzyl group) and of the 2-phthalimidoethyl moiety (introduction of a methyl on the phthalimide substructure, replacement of the phthalimide moiety with a phenyl ring and elongation of the ethyl linker). Most DSs proved to inhibit the wild-type HIV-1 replication in cell-based assays and 15 of them were active at nanomolar concentrations. The most potent congeners (
11,
15,
16,
17,
18,
19,
20 and
32, EC
50: 10–70
nM) shared the
N-
para-substituted phenyl moiety. Compound
17 tested in enzyme assay against recombinant wild-type reverse transcriptase displayed an IC
50 value of 0.74
μM. Compounds
19 and
33 were active at micromolar concentrations against the clinically relevant Y181C and/or K103R resistant mutants. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0968-0896 1464-3391 |
DOI: | 10.1016/j.bmc.2008.05.010 |