Enhanced wound healing effect of canine adipose-derived mesenchymal stem cells with low-level laser therapy in athymic mice

• Published in: Journal of dermatological science Vol. 68; no. 3; pp. 149 - 156
• Main Authors: Kim, Hyoju, Choi, Kyuseok, Kweon, Oh-Kyeong, Kim, Wan Hee
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ireland Ltd 01.12.2012
• Subjects: Adipose Tissue - cytology; Adipose-derived mesenchymal stem cells; Animals; Caspase 3 - metabolism; Cell Differentiation; Cell Survival - radiation effects; Dermatology; Dogs; Female; Fibroblast Growth Factor 2 - secretion; Keratins - metabolism; Ki-67 Antigen - metabolism; Low-level laser therapy; Low-Level Light Therapy; Mesenchymal Stem Cell Transplantation; Mesenchymal Stromal Cells - physiology; Mesenchymal Stromal Cells - radiation effects; Mice; Mice, Nude; Mouse; Skin - injuries; Skin - pathology; Skin - radiation effects; Skin regeneration; Tissue Engineering; Vascular Endothelial Growth Factor A - secretion; Wound healing; Wound Healing - physiology; Wound Healing - radiation effects; Low-level laser therapy; Adipose-derived mesenchymal stem cells; Wound healing; Skin regeneration; Mouse
• ISSN: 0923-1811; 1873-569X
• DOI: 10.1016/j.jdermsci.2012.09.013

Abstract Background Adipose-derived mesenchymal stem cells (ASCs) are attractive cell source for skin tissue engineering. However, one obstacle to this approach is that the transplanted ASC population can decline rapidly in the recipient tissue. Objective The aim of this study was to investigate the effects of low-level laser therapy (LLLT) on transplanted canine ASCs in a skin wound animal model. Methods LLLT, ASC transplantation (ASCs) and ASC transplantation with LLLT (ASCs + LLLT) were applied to the wound bed in athymic mice. Wound healing was assessed by gross evaluation and by hematoxylin and eosin staining. The survival, differentiation and secretion of vascular endothelial growth factor and basic fibroblast growth factor of the ASCs were evaluated by immunohistochemistry and Western blotting. Results The ASCs and ASCs + LLLT groups stimulated wound closure and histological skin regeneration. The ASCs + LLLT group enhanced the wound healing, including neovascularization and regeneration of skin appendages, compared with the ASCs group. The ASCs contributed skin regeneration via differentiation and secretion of growth factors. In the ASCs + LLLT group, the survival of ASCs was increased by the decreased apoptosis of ASCs in the wound bed. The secretion of growth factors was stimulated in the ASCs + LLLT group compared with the ASCs group. Conclusion These data suggest that LLLT is an effective biostimulator of ASCs in wound healing that enhances the survival of ASCs and stimulates the secretion of growth factors in the wound bed.