Next generation sequencing yields complete mitogenomes of Leopard whipray (Himantura leoparda) and Blue-spotted stingray (Neotrygon kuhlii) (Chondrichthyes: Dasyatidae)

The Leopard whipray (Himantura leoparda) and Blue-spotted stingray (Neotrygon kuhlii) are distributed in the Indian and West Pacific Ocean and considered as complex species based on morphological and molecular evidences. In this study, we used the next-generation sequencing method to decode two comp...

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Published inMitochondrial DNA. Part A. DNA mapping, sequencing, and analysis Vol. 27; no. 4; pp. 2613 - 2614
Main Authors Shen, Kang-Ning, Chang, Chih-Wei, Tsai, Shiou-Yi, Wu, Shan-Chun, Lin, Zi-Han, Chan, Yen-Fan, Chen, Ching-Hung, Hsiao, Chung-Der, Borsa, Philippe
Format Journal Article
LanguageEnglish
Published England Taylor & Francis 01.01.2016
Taylor and Francis
Subjects
Animals
Base Composition - genetics
Blue-spotted stingray
Genetics
Genome, Mitochondrial - genetics
High-Throughput Nucleotide Sequencing - methods
leopard whipray
Life Sciences
mitogenome
next generation sequencing
Panthera - classification
Panthera - genetics
Phylogeny
RNA, Ribosomal - genetics
RNA, Transfer - genetics
Blue-spotted stingray
leopard whipray
mitogenome
next generation sequencing
Leopard whipray
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Summary:The Leopard whipray (Himantura leoparda) and Blue-spotted stingray (Neotrygon kuhlii) are distributed in the Indian and West Pacific Ocean and considered as complex species based on morphological and molecular evidences. In this study, we used the next-generation sequencing method to decode two complete mitogenomes of H. leoparda and N. kuhlii. The assembled mitogenome, consisting lengths of 17,690 bp for H. leoparda and 17,974 bp for N. kuhlii, shows 78% identity to each other. Both mitogenomes follow the typical vertebrate arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region of D-loop. D-loop with the lengths 1931 bp (H. leoparda) and 2243 bp (N. kuhlii) is located between tRNA-Pro and tRNA-Phe. The overall GC content is 40.3% for H. leoparda and 39.8% for N. kuhlii. The complete mitogenome of H. leoparda and N. kuhlii provides essential and important DNA molecular data for further phylogenetic and evolutionary analyses for stingray species complex.
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ISSN:2470-1394
2470-1408
DOI:10.3109/19401736.2015.1041119