Inhibition of cellulose saccharification and glycolignin-attacking enzymes of five lignocellulose-degrading fungi by ferulic acid

• Published in: World journal of microbiology & biotechnology Vol. 12; no. 1; pp. 16 - 21
• Main Authors: ASIEGBU, F. O, PATERSON, A, SMITH, J. E
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 1996
• Subjects: Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Chaetomium cellulolyticum; Fundamental and applied biological sciences. Psychology; Mission oriented research; Phanerochaete chrysosporium; Physiology and metabolism; Pleurotus sajor-caju; Trametes versicolor; Trichoderma harzianum; Cellulose; Coriolus versicolor; Basidiomycetes; Cinnamic acid(4-hydroxy 3-methoxy); Fungi; Trichoderma harzianum; Enzymatic activity; Ascomycetes; Fungi Imperfecti; Inhibition; Thallophyta; Laccase; Biodegradation; Microorganism growth; Enzyme; Saccharification; Lignocellulosics; Chaetomium cellulolyticum; Cellulase; Glycosidases; Phanerochaete chrysosporium; Phenols; Hydrolases; Oxidoreductases; O-Glycosidases
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/bf00327792

At 5 g/l, ferulic acid, a plant cell-wall phenolic, severely repressed growth of the lignocellulose-degrading fungi Trichoderma harzianum, Chaetomium cellulolyticum, Phanaerochaete chrysosporium, Trametes versicolor and Pleurotus sajor-caju. At 0.5 g/l, howerver, it slightly stimulated growth of the latter two organisms. Two classes of extracellular enzymes involved in cellulose and glycolignin breakdown were assayed: cellulases; and phenol oxidases as laccases. All of the strains depolymerized cellulose but two (T. versicolor and P. sajor-caju) also secreted laccases. Laccase-secreting fungal species had normal levels of cellulose saccharification except in the presence of 5 g ferulic acid/l, whereas saccharification by the other strains was suppressed at all concentrations of the phenolic tested.