Reliability of Different Image Analysis Methods for Scanning Laser Doppler Flowmetry

• Published in: Current eye research Vol. 33; no. 5-6; pp. 493 - 499
• Main Authors: Kreis, Andreas J., Nguyen, Thanh, Rogers, Sophie, Wang, Jie Jin, Harazny, Joanna, Michelson, Georg, Omar Farouque, H. M., Wong, Tien Y.
• Format: Journal Article
• Language: English
• Published: England Informa UK Ltd 01.05.2008; Taylor & Francis
• Subjects: Adult; AFFPIA; Blood Flow Velocity - physiology; Blood Volume - physiology; Capillaries - physiology; Cross-Sectional Studies; Female; flowmetry; Humans; image analysis; Image Processing, Computer-Assisted - methods; Laser-Doppler Flowmetry - standards; Male; Middle Aged; Observer Variation; reliability; reproducibility; Reproducibility of Results; Retinal Vessels - physiology
• ISSN: 0271-3683; 1460-2202
• DOI: 10.1080/02713680802069149

Purpose: To assess reliability and reproducibility of different analysis methods for retinal capillary flow, volume, and velocity from scanning laser Doppler flowmetry (SLDF) topography. Material and Methods: SLDF topography analysis using the default retinal flowmeter (HRF) were compared to that using automatic full-field perfusion image analyzer (AFFPIA) and quantified as intraclass correlation coefficients (ICC). Results: The AFFPIA full-field method had the highest reliability, with ICC 0.99 for capillary flow. The reproducibility using the AFFPIA full-field method was high ICC 0.74 for capillary flow. Conclusions: The AFFPIA full-field method is highly reliable and superior to the default HRF software.