Study of mixed Langmuir–Blodgett films of immunoglobulin G/amphiphile and their application for immunosensor engineering

• Published in: Biosensors & bioelectronics Vol. 20; no. 6; pp. 1126 - 1133
• Main Authors: Hou, Yanxia, Tlili, Chaker, Jaffrezic-Renault, Nicole, Zhang, Aidong, Martelet, Claude, Ponsonnet, Laurence, Errachid, Abdelhamid, samitier, Josep, Bausells, Joan
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Lausanne Elsevier B.V 15.12.2004; Elsevier Science; Elsevier
• Subjects: ac impedance; Animals; Antigens - analysis; Antigens - chemistry; Antigens - immunology; Behenic acid; Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biosensors; Biotechnology; Coated Materials, Biocompatible - chemistry; Electric power; Engineering Sciences; Equipment Design; Equipment Failure Analysis; Fundamental and applied biological sciences. Psychology; Immunoassay - instrumentation; Immunoassay - methods; Immunoglobulin G; Immunoglobulin G - analysis; Immunoglobulin G - chemistry; Immunoglobulin G - immunology; Immunosensors; LB films; Membranes, Artificial; Methods. Procedures. Technologies; Octadecylamine; Rabbits; Various methods and equipments; Behenic acid; Immunosensors; LB films; Immunoglobulin G; ac impedance; Octadecylamine; Immunoglobulins; IgG; Amphiphilic compound; Immunosensor; Biosensor; Langmuir Blodgett layer; Impedance; Application; immunoglobulin G; SELF-ASSEMBLED MONOLAYERS; ANTIBODY; immunosensors; OCTADECYLAMINE; MOLECULES; BIOSENSORS; behenic acid; BINDING; ANTIGEN; SURFACES
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2004.05.017

Langmuir–Blodgett (LB) technique appears to be quite suitable for generating biospecific surfaces and it has potential application for fabricating biosensors. In this work, mixed Langmuir–Blodgett films of immunoglobulin G/amphiphile have been transferred onto hydrophobic silver surface previously modified by 1-octadecanethiol (ODT) SAMs. In order to obtain stable LB films, the influences of different parameters – type of amphiphile, surface pressure and pH – on the properties of mixed IgG/amphiphile monolayer, were investigated. Electrochemical properties of the engineered immunosensor have been measured by impedimetric spectroscopy. The immunosensor obtained exhibits a high sensitivity and a good specificity in a linear dynamic range from 200 to 1000 ng ml −1.