Crystal structure of a yeast TBP/TATA-box complex

• Published in: Nature (London) Vol. 365; no. 6446; pp. 512 - 520
• Main Authors: Kim, Y, Geiger, J.H, Hahn, S, Sigler, P.B
• Format: Journal Article
• Language: English
• Published: London Nature Publishing 07.10.1993; Nature Publishing Group
• Subjects: Amino Acid Sequence; Base Sequence; Biochemistry; Biological and medical sciences; Computer Graphics; Crystallography, X-Ray; Cytochrome c Group - genetics; Cytochromes c; DNA; DNA - chemistry; Fundamental and applied biological sciences. Psychology; genetic techniques and protocols; Genetics; Hydrogen Bonding; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Nucleic Acid Conformation; Promoter Regions, Genetic; Protein Binding; Protein Conformation; Proteins; Recombinant Proteins - chemistry; Saccharomyces cerevisiae - chemistry; Saccharomyces cerevisiae Proteins; Structure-Activity Relationship; TATA Box; Transcription Factor TFIIA; Transcription Factor TFIID; Transcription Factors - chemistry; Transcription Factors - metabolism; Transcription. Transcription factor. Splicing. Rna processing; Yeast; Yeasts; Fungi; Yeast; Molecular structure; DNA binding protein; TATA box binding protein; Molecular interaction; Ascomycetes; Molecular complex; Saccharomyces cerevisiae; Spatial structure; Thallophyta; Conformation
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/365512a0

The 2.5 angstrom crystal structure of a TATA-box complex with yeast TBP shows that the eight base pairs of the TATA box bind to the concave surface of TBP by bending towards the major groove with unprecedented severity. This produces a wide open, underwound, shallow minor groove which forms a primarily hydrophobic interface with the entire under-surface of the TBP saddle. The severe bend and a positive writhe radically alter the trajectory of the flanking B-form DNA.