Evaluation of immuno-modulating effect of recombinant heat shock protein 40 of Brucella abortus in mice
The present study was aimed to evaluate the immuno-modulatory effect of Brucella -specific recombinant HSP40 (rDnaJ) when co-immunized with Brucella rOmp22 in mice. For this, dnaJ of Brucella abortus was cloned, expressed in E. coli, and purified to homogeneity using Ni–NTA agarose columns. Three gr...
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Published in | 3 Biotech Vol. 9; no. 10; pp. 1 - 8 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Cham
Springer International Publishing
01.10.2019
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | The present study was aimed to evaluate the immuno-modulatory effect of
Brucella
-specific recombinant HSP40 (rDnaJ) when co-immunized with
Brucella
rOmp22 in mice. For this,
dnaJ
of
Brucella abortus
was cloned, expressed in
E. coli,
and purified to homogeneity using Ni–NTA agarose columns. Three groups of mice (
n
= 6 in each group) were used in the study. The control group was immunized with rOmp22 alone, while group 1 mice were injected subcutaneously with rOmp22 along with conventional adjuvants (FCA, FIA), and group 2 mice with rOmp22 mixed with rDnaJ. IgG isotype (IgG1 and IgG2a) response to rOmp22 immunization was evaluated by enzyme-linked immunosorbent assay which was found to be directed towards the cell-mediated arm of immune system (CMI) in group 2 mice in which rOmp22 was co-immunized with rDnaJ. Expression profiling of IL-4 and IL-12 was checked in all the groups by qRT PCR. IL12 mRNA was up-regulated to a greater extent in group2 mice, suggesting that the CMI arm of immune system was stimulated. Hence, it was concluded that CMI response against rOmp22 is stimulated to a greater extent in mice when co-immunized with
Brucella
rDnaJ. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2190-572X 2190-5738 |
DOI: | 10.1007/s13205-019-1905-3 |