Evaluation of immuno-modulating effect of recombinant heat shock protein 40 of Brucella abortus in mice

• Published in: 3 Biotech Vol. 9; no. 10; pp. 1 - 8
• Main Authors: Minhas, Priyanka, Sunil Kumar, B. V., Verma, Ramneek
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.10.2019; Springer Nature B.V
• Subjects: Adjuvants; Agriculture; Bioinformatics; Biomaterials; Biotechnology; Brucella; Brucella abortus; Cancer Research; Chemistry; Chemistry and Materials Science; E coli; Enzyme-linked immunosorbent assay; Heat shock proteins; Hsp40 protein; Immune system; Immunization; Immunoglobulin G; Interleukin 1; Interleukin 12; Interleukin 4; mRNA; Original; Original Article; Stem Cells; DnaJ; rOmp22; Heat Shock Proteins; Immuno-modulatory
• ISSN: 2190-572X; 2190-5738
• DOI: 10.1007/s13205-019-1905-3

The present study was aimed to evaluate the immuno-modulatory effect of Brucella -specific recombinant HSP40 (rDnaJ) when co-immunized with Brucella rOmp22 in mice. For this, dnaJ of Brucella abortus was cloned, expressed in E. coli, and purified to homogeneity using Ni–NTA agarose columns. Three groups of mice ( n  = 6 in each group) were used in the study. The control group was immunized with rOmp22 alone, while group 1 mice were injected subcutaneously with rOmp22 along with conventional adjuvants (FCA, FIA), and group 2 mice with rOmp22 mixed with rDnaJ. IgG isotype (IgG1 and IgG2a) response to rOmp22 immunization was evaluated by enzyme-linked immunosorbent assay which was found to be directed towards the cell-mediated arm of immune system (CMI) in group 2 mice in which rOmp22 was co-immunized with rDnaJ. Expression profiling of IL-4 and IL-12 was checked in all the groups by qRT PCR. IL12 mRNA was up-regulated to a greater extent in group2 mice, suggesting that the CMI arm of immune system was stimulated. Hence, it was concluded that CMI response against rOmp22 is stimulated to a greater extent in mice when co-immunized with Brucella rDnaJ.