PSHG-TISS: A collection of polarization-resolved second harmonic generation microscopy images of fixed tissues

• Published in: Scientific data Vol. 9; no. 1; p. 376
• Main Authors: Hristu, Radu, Stanciu, Stefan G., Dumitru, Adrian, Eftimie, Lucian G., Paun, Bogdan, Tranca, Denis E., Gheorghita, Pavel, Costache, Mariana, Stanciu, George A.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 02.07.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/1647/245/2226; 631/67/2321; Collagen; Collagen (type I); Confocal microscopy; Data Descriptor; Humanities and Social Sciences; Image processing; Microscopy; multidisciplinary; Polarization; Science; Science (multidisciplinary)
• ISSN: 2052-4463; 2052-4463
• DOI: 10.1038/s41597-022-01477-1

Second harmonic generation (SHG) microscopy is acknowledged as an established imaging technique capable to provide information on the collagen architecture in tissues that is highly valuable for the diagnostics of various pathologies. The polarization-resolved extension of SHG (PSHG) microscopy, together with associated image processing methods, retrieves extensive image sets under different input polarization settings, which are not fully exploited in clinical settings. To facilitate this, we introduce PSHG-TISS, a collection of PSHG images, accompanied by additional computationally generated images which can be used to complement the subjective qualitative analysis of SHG images. These latter have been calculated using the single-axis molecule model for collagen and provide 2D representations of different specific PSHG parameters known to account for the collagen structure and distribution. PSHG-TISS can aid refining existing PSHG image analysis methods, while also supporting the development of novel image processing and analysis methods capable to extract meaningful quantitative data from the raw PSHG image sets. PSHG-TISS can facilitate the breadth and widespread of PSHG applications in tissue analysis and diagnostics. Measurement(s) Type I Collagen Technology Type(s) multi-photon laser scanning microscopy Factor Type(s) second order susceptibility tensor elements Sample Characteristic - Organism Homo sapiens Sample Characteristic - Environment laboratory environment Sample Characteristic - Location Romania