A Novel Enzyme-Free Biosensor Based on Porous Core-Shell Metal Organic Frame Nanocomposites Modified Electrode for Highly Sensitive Detection of Uric Acid and Dopamine

• Published in: Journal of biomedical nanotechnology Vol. 15; no. 7; p. 1443
• Main Authors: Qiu, Ruiqi, Xu, Qiu, Jiang, Hui, Wang, Xuemei
• Format: Journal Article
• Language: English
• Published: United States 01.07.2019
• Subjects: Ascorbic Acid; Biosensing Techniques; Dopamine; Electrodes; Humans; Metals; Nanocomposites; Uric Acid
• ISSN: 1550-7033
• DOI: 10.1166/jbn.2019.2791

A novel enzyme-free biosensor based on porous metal organic frame nanocomposites, i.e., core-shell structured Au@NC@GC nanocomposites, has been constructed for simultaneous determination of uric acid (UA) and dopamine (DA). Au@ZIF-8@ZIF-67 was prepared through a seed-mediated growth method and carbonized in nitrogen atmosphere to synthesize a nanoporous hybrid carbon materials (Au@NC@GC). Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) studies demonstrate that the as-prepared Au@NC@GC modified glassy carbon electrode (Au@NC@GC-GCE) possesses a high selectivity and sensitivity for simultaneous detections of UA and DA. It exhibited wide linear responses for UA and DA in the range from 10 M to 600 M and 10 M to 150 M, with the detection limits of 0.773 nM and 0.746 nM, respectively (S/N = 3). Moreover, this novel electrochemical biosensor could be further utilized in biological analysis (i.e., human serum), and the satisfactory recovery results of UA and DA could be readily obtained. These afore-mentioned results further manifest that the as-prepared biosensors are capable for quantitatively monitoring UA and DA in serum, verifying the possibility for its future promising applications in real biological or clinic samples analysis.