HIV-1 mRNA knockdown with CRISPR/CAS9 enhances neurocognitive function

• Published in: Journal of neurovirology Vol. 30; no. 1; pp. 71 - 85
• Main Authors: McLaurin, Kristen A., Li, Hailong, Khalili, Kamel, Mactutus, Charles F., Booze, Rosemarie M.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.02.2024
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; CRISPR-Cas Systems; Gene Editing - methods; HIV Seropositivity; HIV-1 - genetics; Humans; Immunology; Infectious Diseases; Neurology; Neurosciences; Rats; RNA, Messenger - genetics; Virology; Cell culture; In situ hybridization; Gene editing; Microglia; Prepulse inhibition

Mixed glia are infiltrated with HIV-1 virus early in the course of infection leading to the development of a persistent viral reservoir in the central nervous system. Modification of the HIV-1 genome using gene editing techniques, including CRISPR/Cas9, has shown great promise towards eliminating HI...