Gills specific type 2 crustin isoforms: Its molecular cloning and characterization from kuruma shrimp Marsupenaeus japonicus

• Published in: Developmental and comparative immunology Vol. 85; pp. 25 - 30
• Main Authors: Tandel, Gauravkumar M., Kondo, Hidehiro, Hirono, Ikuo
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01.08.2018; Elsevier Science Ltd
• Subjects: Antimicrobial peptides; Antimicrobial peptides (AMPs); Bacteria; Cloning; Crustaceans; Crustin isoform; Experimental infection; Gene expression; Gills; Gills specific; Gram-negative bacteria; Hemocytes; Isoforms; Marsupenaeus japonicus; Molecular chains; Nucleotide sequence; Penaeus japonicus; Peptides; Phylogeny; Proteins; Shellfish; Tissue analysis; Vibrio parahaemolyticus; Viruses; Waterborne diseases; White spot syndrome; WSSV; Antimicrobial peptides (AMPs); Crustin isoform; Vibrio parahaemolyticus; Gills specific; WSSV
• ISSN: 0145-305X; 1879-0089
• DOI: 10.1016/j.dci.2018.03.018

Crustins are diverse group of antimicrobial peptides (AMPs) that have numerous isoforms mainly identified from hemocytes in decapods crustacean. However, little is known about its presence solely in gills tissue. In this study, we found two new crustin isoforms MjCRS8 and MjCRS9 by using transcriptome analysis from gills. Open reading frame of MjCRS8 and MjCRS9 were 593 bp and 459 bp encoding 197aa and 152aa, respectively. Tissue distribution analysis indicated that both MjCRS8 and MjCRS9 are expressed only in gills tissue. Multiple sequence alignment and phylogenetic analysis with previously reported crustin suggested that both MjCRS8 and MjCRS9 belong to type 2 crustin family. Experimental infection was conducted against Vibrio parahaemolyticus and white spot syndrome virus (WSSV) by immersion test. However, no significant upregulation was observed. •Two new isoforms of crustin MjCRS8 and MjCRS9 were identified from kuruma shrimp M. japonicus.•Interestingly both MjCRS8 and MjCRS9 are gills specific.•Multiple sequence alignment and phylogenetic analysis classify MjCRS8 and MjCRS9 as type 2 crustin family.•Both MjCRS8 and MjCRS9 were not significantly upregulated against V. parahaemolyticus and WSSV challenge test.