Identification and characterization of a fimbrial gene cluster of Edwardsiella tarda expressing mannose-resistant hemagglutination

We determined the nucleotide sequence of a 8.6 kb DNA region containing etfA encoding a putative fimbrial major subunit from chromosomal DNA of Edwardsiella tarda KG8401 which expresses mannose-resistant hemagglutination (MRHA). This region contained three novel genes, etfBCD, at the downstream regi...

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Bibliographic Details
Published inFish Pathology Vol. 39; no. 2; pp. 87 - 93
Main Authors Sakai, T. (Nagasaki Univ. (Japan)), Kanai, K, Osatomi, K, Yoshikoshi, K
Format Journal Article
LanguageEnglish
Published The Japanese Society of Fish Pathology 01.06.2004
Subjects
EDWARDSIELLA
Edwardsiella tarda
Escherichia coli
fimbriae
gene cluster
GENES
HAEMAGGLUTINATION TESTS
MANNOSE
mannose-resistant hemagglutination
NUCLEOTIDE SEQUENCE
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Summary:We determined the nucleotide sequence of a 8.6 kb DNA region containing etfA encoding a putative fimbrial major subunit from chromosomal DNA of Edwardsiella tarda KG8401 which expresses mannose-resistant hemagglutination (MRHA). This region contained three novel genes, etfBCD, at the downstream region of etfA. The deduced amino acid sequences of EtfABCD contained conserved fimbrial domains; fimbrial protein, fimbrial chaperone, fimbrial usher and fimbrial protein, respectively. Escherichia coli transformed with the cloned etf operon expressed MRHA and fimbriae that reacted with rabbit antiserum against the fimbrial major subunit of E. tarda, showing the implication of the fimbriae in the hemagglutination of E. tarda.
Bibliography:L73
2005001670
L10
ObjectType-Article-1
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ISSN:0388-788X
1881-7335
DOI:10.3147/jsfp.39.87