The m6A-methylase complex recruits TREX and regulates mRNA export

• Published in: Scientific reports Vol. 8; no. 1; pp. 1 - 12
• Main Authors: Lesbirel, Simon, Viphakone, Nicolas, Parker, Matthew, Parker, Jacob, Heath, Catherine, Sudbery, Ian, Wilson, Stuart A.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 14.09.2018; Nature Publishing Group
• Subjects: 13; 13/89; 38/1; 38/39; 45; 631/337/1645/1792; 631/337/1645/2052; Gene expression; Humanities and Social Sciences; Methylase; mRNA guanylyltransferase; multidisciplinary; N6-methyladenosine; Proteins; RNA modification; Science; Science (multidisciplinary); Splicing; Transcription; Writers; Methylase Complex; Trexate; Export Block; mRNA Export; Top CH
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-32310-8

N 6 -methyladenosine (m 6 A) is the most abundant internal modification of eukaryotic mRNA. This modification has previously been shown to alter the export kinetics for mRNAs though the molecular details surrounding this phenomenon remain poorly understood. Recruitment of the TREX mRNA export complex to mRNA is driven by transcription, 5′ capping and pre-mRNA splicing. Here we identify a fourth mechanism in human cells driving the association of TREX with mRNA involving the m 6 A methylase complex. We show that the m 6 A complex recruits TREX to m 6 A modified mRNAs and this process is essential for their efficient export. TREX also stimulates recruitment of the m 6 A reader protein YTHDC1 to the mRNA and the m 6 A complex influences the interaction of TREX with YTHDC1. Together our studies reveal a key role for TREX in the export of m 6 A modified mRNAs.