Biopolymer-based membranes associated with osteogenic growth peptide for guided bone regeneration

• Published in: Biomedical materials (Bristol) Vol. 13; no. 3; p. 035009
• Main Authors: Saska, Sybele, Pigossi, Suzane C, Oliveira, Guilherme J P L, Teixeira, Lucas N, Capela, Marisa V, Gonçalves, Andreia, de Oliveira, Paulo T, Messaddeq, Younès, Ribeiro, Sidney J L, Gaspar, Ana Maria Minarelli, Marchetto, Reinaldo
• Format: Journal Article
• Language: English
• Published: England IOP Publishing 14.03.2018
• Subjects: Animals; Animals, Newborn; bacterial cellulose; barrier membrane; Biopolymers - chemistry; Bone and Bones - pathology; Bone Regeneration; Cell Culture Techniques; Cell Membrane - metabolism; Cell Proliferation; collagen; Collagen - chemistry; Histones - chemistry; Intercellular Signaling Peptides and Proteins - chemistry; Male; Osteoblasts - cytology; Osteogenesis - drug effects; Peptides - chemistry; Polymers - chemistry; Rats; Rats, Wistar
• ISSN: 1748-605X; 1748-605X
• DOI: 10.1088/1748-605X/aaaa2d

Barrier membranes for guided bone regeneration (GBR) mainly promote mechanical maintenance of bone defect space and induce osteopromotion. Additionally, biopolymer-based membranes may provide greater bioactivity and biocompatibility due to their similarity to extracellular matrix (ECM). In this study, biopolymers-based membranes from bacterial cellulose (BC) and collagen (COL) associated with osteogenic growth peptide (OGP(10-14)) were evaluated to determine in vitro osteoinductive potential in early osteogenesis; moreover, histological study was performed to evaluate the BC-COL OGP(10-14) membranes on bone healing after GBR in noncritical defects in rat femur. The results showed that the BC-COL and BC-COL OGP(10-14) membranes promoted cell proliferation and alkaline phosphatase activity in osteoblastic cell cultures. However, ECM mineralization was similar between cultures grown on BC OGP(10-14) and BC-COL OGP(10-14) membranes. In vivo results showed that all the membranes tested, including the peptide-free BC membrane, promoted better bone regeneration than control group. Furthermore, the BC-COL OGP(10-14) membranes induced higher radiographic density in the repaired bone than the other groups at 1, 4 and 16 weeks. Histomorphometric analyses revealed that the BC-COL OGP(10-14) induced higher percentage of bone tissue in the repaired area at 2 and 4 weeks than others membranes. In general, these biopolymer-based membranes might be potential candidates for bone regeneration applications.