Expression of pituitary adenylate cyclase-activating polypeptide 1 and 2 receptor mRNA in gallbladder tissue of patients with gallstone or gallbladder polyps

• Published in: World journal of gastroenterology : WJG Vol. 12; no. 9; pp. 1468 - 1471
• Main Author: Zhang, Zhen-Hai
• Format: Journal Article
• Language: English
• Published: United States No.2 Department of General Surgery, Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China%No.2 Department of General Surgery, Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China 07.03.2006; Key Laboratory of Congenital Malformation of Public Health Ministry, Second Affiliated Hospital, China Medical University,Shenyang 110004,Liaoning Province,China; Baishideng Publishing Group Co., Limited
• Subjects: Adult; Female; Gallbladder - chemistry; Gallbladder Diseases - genetics; Gallbladder Diseases - metabolism; Gallstones - chemistry; Gallstones - etiology; Gallstones - genetics; Gene Expression Regulation; Humans; Male; Middle Aged; mRNA; Polyps - chemistry; Polyps - etiology; Polyps - genetics; Rapid Communication; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I - genetics; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I - physiology; Receptors, Vasoactive Intestinal Polypeptide, Type I - genetics; Receptors, Vasoactive Intestinal Polypeptide, Type I - physiology; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; 多肽; 环化酶; 胆囊疾病; 胆石症; 黏液腺苷酸; VPCAP2-R; VPCAP1-R; Gallbladder disease; RT-PCR
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v12.i9.1468

AIM： To detect the expression of pituitary adenylate cyclase-activating polypeptide receptor i （VPCAPrR） and VPCAP2-R mRNA in gallbladder tissues of patients with gallstone or gallbladder polyps. METHODS： The expression of VPCAP1-R and VPCAP2-R mRNA in gallbladder tissues was detected in 25 patients with gallstone, 8 patients with gallbladder polyps and 7 donors of liver transplantation by reverse transcription polymerase chain reaction （RT-PCR）. RESULTS： The VPCAP2-R mRNA expression level in the control group （1.09±0.58） was lower than that in the gallbladder polyp group （1.64±0.56） and the gallstone group （1.55±0.45） （P〈0.05） while the VPCAP1-R mRNA expression level in the control group （1.15 ±0.23） was not apparently different from that in the gallbladder polyp group （1.28±0.56） and the gallstone group （1.27± 0.38）. CONCLUSION： The abnormal expression of VPCAP2-R mRNA in gallbladder tissue may play a role in the formation of gallbladder stone and gallbladder polyps.