Development of surface-functionalised nanoparticles for FGF2 receptor-based solid tumour targeting

• Published in: Journal of microencapsulation Vol. 29; no. 1; pp. 95 - 102
• Main Authors: Jain, Amit, Gulbake, Arvind, Jain, Ashish, Shilpi, Satish, Hurkat, Pooja, Jain, Aviral, Jain, Sanjay K.
• Format: Journal Article
• Language: English
• Published: Colchester Informa UK, Ltd 01.01.2012; Taylor & Francis; Informa
• Subjects: Biological and medical sciences; Cell Line, Tumor; cisplatin; Drug Delivery Systems; FGF2 receptor; Fibroblast Growth Factor 2 - metabolism; gelatin nanoparticles; General pharmacology; heparin; Humans; Medical sciences; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Microscopy, Fluorescence; Nanoparticles; Neoplasms - drug therapy; Neoplasms - metabolism; Particle Size; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Receptors, Fibroblast Growth Factor - metabolism; Surface Properties; Tissue Distribution; tumour targeting; Antineoplastic agent; Solid tumor; Pharmaceutical technology; Targeting; Nanoparticle; tumour targeting; Anticoagulant; Malignant tumor; Cisplatin; Polyelectrolyte; Alkylating agent; Target; Gelatin; Animal protein; Glycosaminoglycan; Microparticle; Oside polymer; FGF2 receptor; Heparin; gelatin nanoparticles; Cancer; Platinum II Complexes; Biological receptor
• ISSN: 0265-2048; 1464-5246
• DOI: 10.3109/02652048.2011.635219

The surface-functionalised gelatin nanoparticles (GNPs) containing cisplatin were developed and characterised for breast cancer targeting using fibroblast growth factor-2 (FGF2) receptors which are overexpressed on breast cancer cells. The GNPs were prepared using two-step desolvation method and then the surface of GNPs was functionalised with activated heparin. They were characterised for surface morphology, particle size and size distribution, surface charge, entrapment efficiency and in vitro drug release. The results revealed that the mean diameter of GNPs was 173 ± 2.2 nm with smooth surface, which was increased to 189 ± 3.4 nm after coupling with heparin (H-GNPs). The targeting effect of H-GNPs and GNPs was investigated by in vitro cell uptake study on human breast cancer MDA-MB-231 cell line, which exhibited greater uptake of H-GNPs as compared to GNPs. Therefore, it is suggested that H-GNPs can be used as an effective carrier for solid tumour targeting.