HIF-2α affects proliferation and apoptosis of MG-63 osteosarcoma cells through MAPK signaling

• Published in: Molecular medicine reports Vol. 15; no. 4; pp. 2174 - 2178
• Main Authors: Wang, Yuqiang, Wang, Xiaohua, Su, Xuetao, Liu, Tiansheng
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.04.2017; Spandidos Publications UK Ltd
• Subjects: Angiogenesis; Apoptosis; Basic Helix-Loop-Helix Transcription Factors - genetics; Basic Helix-Loop-Helix Transcription Factors - metabolism; Bone and Bones - metabolism; Bone and Bones - pathology; Bone cancer; Bone Neoplasms - genetics; Bone Neoplasms - metabolism; Bone Neoplasms - pathology; Cancer; Cell cycle; Cell Line, Tumor; Cell Proliferation; Flow cytometry; HIF-2α; Humans; Hypoxia; Hypoxia-inducible factors; Kinases; MAP kinase; MAP Kinase Signaling System; Medical prognosis; Metastasis; Osteosarcoma; Osteosarcoma - genetics; Osteosarcoma - metabolism; Osteosarcoma - pathology; Osteosarcoma cells; p38 Mitogen-Activated Protein Kinases - metabolism; Protein kinase; Proteins; RNA Interference; RNA, Small Interfering - genetics; Sarcoma; Signal transduction; siRNA; Software; Statistical analysis; Studies; Transfection; Western blotting; United States > US; China
• ISSN: 1791-2997; 1791-3004
• DOI: 10.3892/mmr.2017.6243

The present study explored the mechanism of hypoxia-inducible factor (HIF)-2α in proliferation and apoptosis of the osteosarcoma cell line, MG-63. Cells were treated with small interfering RNA (siRNA) against HIF-2α (silenced group) or without siRNA (control group). Cell viability of MG-63 in the silenced and the control groups was determined by MTT assay; cell apoptosis was measured by flow cytometry; the expression of HIF-2α and mitogen-activated protein kinase (MAPK)-p38 were measured by western blotting. According to MTT assay, 48 h after siRNA transfection, compared with the control group, cells in the silenced group significantly declined in quantity and the number of apoptotic cells increased significantly. The expression of HIF-2α and MAPK-p38 were significantly decreased (P<0.05). In conclusion, knockdown of HIF-2α in the osteosarcoma cell line reduced the proliferation of cancer cells and increased apoptosis. These effects likely occurred through the MAPK-p38 signaling pathway.