Effects of embryonic cadmium exposure on erythrocyte indices and morphology in newly hatched Gallus gallus domesticus chicks

• Published in: Poultry science Vol. 101; no. 6; p. 101862
• Main Authors: Bojarski, Bartosz, Chmurska-Gąsowska, Maria, Gałuszka, Anna, Kozłowska, Anna, Kotula-Balak, Małgorzata, Trela, Magdalena, Kirpaniova, Alena, Kustra, Kamil, Stonawski, Bartłomiej, Łapiński, Stanisław, Arent, Zbigniew, Lis, Marcin W.
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.06.2022; Elsevier
• Subjects: bird; erythrogram; heavy metal; hematology; PHYSIOLOGY AND REPRODUCTION; spectrin; erythrogram; heavy metal; hematology; spectrin; bird
• ISSN: 0032-5791; 1525-3171
• DOI: 10.1016/j.psj.2022.101862

The aim of the current study was to assess the influence of embryonic exposure to cadmium on basic and derived erythrocyte indices, the morphology and morphometric properties of erythrocytes, as well as erythrocyte spectrin distribution in newly hatched Gallus gallus domesticus chicks. The eggs were injected with cadmium (Cd) at a dose of 2 µg, 4 µg, 6 µg, or 8 µg per egg on the sixth day of incubation. Blood samples were collected on the first day after hatching. Exposure to cadmium resulted in higher levels of red blood cell count, hemoglobin concentration, and hematocrit value, while derived erythrocyte indices were lower (mean corpuscular volume) or higher (mean corpuscular hemoglobin concentration) in comparison to the control. These changes occurred in animals exposed to higher doses of this toxic agent. In cadmium-treated individuals (2 and 8 µg of Cd), the percentage of erythrocytes which exhibited changed shape increased. Increases in the length (6 and 8 µg) and width (2, 6, and 8 µg) of erythrocytes and the length and width of the nucleus (2–8 µg) of red blood cells were observed. Changes in spectrin distribution were also observed, which indicate alterations at structural and molecular levels.