Distinct Role of Core Promoter Architecture in Regulation of Light-Mediated Responses in Plant Genes

• Published in: Molecular plant Vol. 7; no. 4; pp. 626 - 641
• Main Authors: Srivastava, Rakesh, Rai, Krishan Mohan, Srivastava, Meenal, Kumar, Verandra, Pandey, Bindu, Singh, Sudhir P., Bag, Sumit K., Singh, Brahma Deo, Tuli, Rakesh, Sawant, Samir V.
• Format: Journal Article
• Language: English
• Published: UK Elsevier Inc 01.04.2014; Oxford University Press; Cell Press
• Subjects: Arabidopsis - genetics; Architecture; cis-regulatory elements; Gene expression; initiator element; Light; nucleosome; Nucleosomes - metabolism; nucleotide polymorphism; Promoter Regions, Genetic - genetics; TATA-box; TATA盒; transcription; Transcription, Genetic; 介导; 反应; 子结构; 核心启动子; 植物基因; 调控作用; 转录起始位点; TATA-box; nucleotide polymorphism; cis-regulatory elements; initiator element; transcription; light; nucleosome; regulatory elements; box
• ISSN: 1674-2052; 1752-9867
• DOI: 10.1093/mp/sst146

In the present study, we selected four distinct classes of light-regulated promoters. The light-regulated promoters can be distinctly grouped into either TATA-box-containing or TATA-less （initiator-containing） promoters. Further, using either native promoters or their swapped versions of core promoter elements, we established that TATA-box and Inr （Initiator） elements have distinct mechanisms which are involved in light-mediated regulation, and these elements are not swappable. We identified that mutations in either functional TATA-box or Inr elements lead to the formation of nucleosomal structure. The nucleotide diversity in either the TATA-box or Inr element in Arabidopsis ecotypes proposes that the nucleotide variation in core promoters can alter the gene expression. We show that motif overrepresentation in light-activated promoters encompasses different specific regulatory motifs present downstream of TSS （transcription start site）, and this might serve as a key factor in regulating light promoters which are parallel with these elements. Finally, we conclude that the TATA-box or Inr element does not act in isolation, but our results clearly suggests the probable involvement of other distinct core promoter elements in concurrence with the TATA-box or Inr element to impart selectivity to light-mediated transcription.