Label-Free Electronic Detection of Thrombin in Blood Serum by Using an Aptamer-Based Sensor

Blue, gold, and DNA: A methylene blue (MB) tagged, thrombin‐binding DNA aptamer immobilized on a gold surface undergoes a large conformational change upon target binding (see schematic representation; eT: electron transfer). This folding produces a large, readily measurable change in redox current a...

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Bibliographic Details
Published inAngewandte Chemie International Edition Vol. 44; no. 34; pp. 5456 - 5459
Main Authors Xiao, Yi, Lubin, Arica A., Heeger, Alan J., Plaxco, Kevin W.
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 26.08.2005
WILEY‐VCH Verlag
Subjects
aptamers
Aptamers, Nucleotide - chemistry
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
biosensors
DNA structures
Electrochemistry
electron transfer
Humans
Models, Biological
Sensitivity and Specificity
thrombin
Thrombin - analysis
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Summary:Blue, gold, and DNA: A methylene blue (MB) tagged, thrombin‐binding DNA aptamer immobilized on a gold surface undergoes a large conformational change upon target binding (see schematic representation; eT: electron transfer). This folding produces a large, readily measurable change in redox current and allows the electrochemical detection of thrombin in blood serum.
Bibliography:ArticleID:ANIE200500989
istex:38238F2C898C840CC37B7AFD9BFE92D937435A52
This work was supported in part by the National Institutes of Health (Grant no.: EB002046) and by the Institute for Collaborative Biotechnologies (Grant no.: DAAD19-03-D-0004) from the U.S. Army Research Office. We gratefully acknowledge Brian Baker and Kevin Cash for providing critical commentary and Professor Pierre Petroff for his support.
ark:/67375/WNG-WKG00HFW-B
This work was supported in part by the National Institutes of Health (Grant no.: EB002046) and by the Institute for Collaborative Biotechnologies (Grant no.: DAAD19‐03‐D‐0004) from the U.S. Army Research Office. We gratefully acknowledge Brian Baker and Kevin Cash for providing critical commentary and Professor Pierre Petroff for his support.
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ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200500989