Expression of Ventricular Myosin Subunits in the Atria of Children With Congenital Heart Malformations

The presence of ventricular myosin light chains in the atria of children with congenital heart disease was demonstrated by two-dimensional polyacrylamide gel electrophoresis, peptide mapping, and Western blot analysis. Ventricular myosin light chains were present in 27% of biopsies from 91 children...

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Published inCirculation research Vol. 69; no. 6; pp. 1601 - 1607
Main Authors Shi, Qinwei, Danilczyk, Ursula, Wang, Jinxia, See, Yew P, Williams, William G, Trusler, George A, Beaulieu, R, Rose, Vera, Jackowski, George
Format Journal Article
LanguageEnglish
Published Hagerstown, MD American Heart Association, Inc 01.12.1991
Lippincott
Subjects
Biological and medical sciences
Blood Pressure
Blotting, Western
Cardiology. Vascular system
Congenital heart diseases. Malformations of the aorta, pulmonary vessels and vena cava
Electrophoresis, Gel, Two-Dimensional
Heart
Heart Atria - chemistry
Heart Defects, Congenital - physiopathology
Heart Ventricles - chemistry
Humans
Medical sciences
Myosins - chemistry
Peptide Mapping
Heart
Human
Congenital
Gel electrophoresis
Heart disease
Myosin
Immunoblotting assay
Contractile protein
Cardiovascular disease
Gene expression
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Summary:The presence of ventricular myosin light chains in the atria of children with congenital heart disease was demonstrated by two-dimensional polyacrylamide gel electrophoresis, peptide mapping, and Western blot analysis. Ventricular myosin light chains were present in 27% of biopsies from 91 children with different forms of congenital heart disease. Perimembranous ventricular septal defects and tetralogy of Fallot were associated with the presence of ventricular myosin light chains in 50% of patients. The presence of ventricular myosin light chains in these atria did not correlate with pressure or volume overload. Analysis of myosin heavy chain isotype in the same biopsies by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, peptide mapping, and Western blot analysis indicated that there was no detectable expression of ventricular myosin heavy chain (β-subunit), suggesting that the genes for the myosin heavy chains and light chains are not expressed coordinately.
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ISSN:0009-7330
1524-4571
DOI:10.1161/01.RES.69.6.1601