Interaction of the taxilin family with the nascent polypeptide‐associated complex that is involved in the transcriptional and translational processes
α‐Taxilin is a novel binding partner of the syntaxin family, which is implicated in intracellular vesicle traffic. We have here found that α‐taxilin interacts with the nascent polypeptide‐associated complex (NAC), which is involved in transferring growing nascent polypeptide chains to appropriate co...
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Published in | Genes to cells : devoted to molecular & cellular mechanisms Vol. 10; no. 5; pp. 465 - 476 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Science Ltd
01.05.2005
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Subjects | |
Online Access | Get full text |
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Summary: | α‐Taxilin is a novel binding partner of the syntaxin family, which is implicated in intracellular vesicle traffic. We have here found that α‐taxilin interacts with the nascent polypeptide‐associated complex (NAC), which is involved in transferring growing nascent polypeptide chains to appropriate co‐translationally acting factors. NAC is composed of two subunits, α‐ and βNACs. Both these subunits bound to α‐taxilin through its C‐terminal coiled‐coil region in dose‐dependent and saturable manners. The interactions of α‐taxilin with αNAC and NAC but not with βNAC were inhibited by syntaxin‐4, indicating that α‐taxilin binds to NAC mainly through its interaction with αNAC. When αNAC was over‐expressed in COS‐7 cells, αNAC was distributed in the cytosol and nucleus. However, co‐expression of the α‐taxilin fragment containing the αNAC‐binding region eliminated the nuclear distribution of over‐expressed αNAC. Moreover, other taxilin family members, β‐ and γ‐taxilins, also bound to αNAC and thereby affected the nuclear distribution of over‐expressed αNAC. Taken together with the evidence that αNAC functions in the nucleus as a transcriptional coactivator, our results raise the possibility that the taxilin family is involved not only in the translational process through its interaction with NAC but also in the transcriptional process through its interaction with αNAC alone. |
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Bibliography: | Communicated by Yoshimi Takai ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 1356-9597 1365-2443 |
DOI: | 10.1111/j.1365-2443.2005.00848.x |