Production of asiaticoside from centella(Centella asiatica L.Urban)cells in bioreactor

• Published in: Asian Pacific journal of tropical biomedicine Vol. 3; no. 10; pp. 806 - 810
• Main Authors: Loc, Nguyen Hoang, Nhat, Nguyen Thi Duy
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.10.2013; Asian Pacific Tropical Medicine Press
• Subjects: Anti-Infective Agents - chemistry; Anti-Infective Agents - metabolism; asiatica; Asiaticoside; Basic Researches; Batch Cell Culture Techniques; Bioreactor; Bioreactors; Cell; Cell suspension culture; Centella; Centella - cytology; Centella asiatica; Chromatography, High Pressure Liquid; culture; Plant Cells - metabolism; suspension; Triterpenes - chemistry; Triterpenes - metabolism; Centella asiatica; Bioreactor; Cell suspension culture; Asiaticoside
• ISSN: 2221-1691; 2588-9222
• DOI: 10.1016/S2221-1691(13)60159-8

Objective:To investigate the effects of some culture conditions on production of asialicoside from centella(Centella asiatica L.Urban)cells cultured in 5-L bioreactor.Methods:The centell cell suspension culture was conducted in 5-L bioreactor to investigate the growth and asiaticoside accumulation under various conditions.Asiaticoside content was determined by HPLC analysis.Results:The results showed that the cell growth and asiaticoside accumulation peaked after 24d of culture at an agitation speed of 150 r/min and aeration rate of 2.5 L/min.The cell biomass reached a maximum value of 302.45 g fresh weight(31.43 g dry weight)and growth index of 3.03with inoculum size of 100 g.However,asiaticoside content was the highest(60.08 mg/g dry weight)when culture was initiated with an inoculum size of 50 g.Conclusions:The present study found the suitable conditions for growth of centella cells and their asiaticoside production in bioreactor.