Recent advances in peptide and peptidomimetic stereoisomer separations by capillary electromigration techniques

• Published in: Electrophoresis Vol. 27; no. 1; pp. 222 - 230
• Main Author: Scriba, Gerhard K. E.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.01.2006; WILEY‐VCH Verlag
• Subjects: Capillary electrochromatography; Capillary electrophoresis; Electrophoresis, Capillary - methods; Oligopeptides - chemistry; Oligopeptides - isolation & purification; Peptides; Peptidomimetics; Review; Stereoisomer analysis; Stereoisomerism
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/elps.200500446

As the stereochemistry of peptides determines their physicochemical properties and biological activities, analytical methods able to discriminate between peptide stereoisomers are important especially with regard to pharmaceutical peptides and peptidomimetics. The present review summarizes recent developments in peptide and peptidomimetic stereoisomer separations by capillary electromigration techniques. The majority of separations were performed by CE while only few reports have been published on the subject of electrochromatography. In addition to systematic studies on the applicability of certain buffer additives and the evaluation of specific experimental conditions, there have been attempts to understand the mechanistic aspects of peptide stereoisomer separations as well as to analyze the structure of peptide–CD complexes.