Composite ‘Thymoheart’ Transplantation Improves Cardiac Allograft Survival

• Published in: American journal of transplantation Vol. 4; no. 1; pp. 79 - 86
• Main Authors: Menard, Matthew T., Schwarze, Margaret L., Allan, James S., Johnston, Douglas R., Mawulawde, Kwabena, Shimizu, Akira, Yamada, Kazuhiko, Houser, Stuart L., Allison, Kenneth S., Sachs, David H., Madsen, Joren C.
• Format: Journal Article
• Language: English
• Published: 9600 Garsington Road , Oxford , OX4 2DQ , UK Munksgaard International Publishers 01.01.2004
• Subjects: Animals; Cyclosporine - pharmacology; Flow Cytometry; Haplotypes; Heart; Heart - physiology; Heart Transplantation - methods; Histocompatibility Antigens Class I - immunology; Histocompatibility Antigens Class I - metabolism; Histocompatibility Antigens Class II - immunology; Histocompatibility Antigens Class II - metabolism; Homozygote; Humans; Immunoglobulin G - chemistry; Immunoglobulin M - chemistry; Immunohistochemistry - methods; Immunosuppression Therapy; Immunosuppressive Agents - pharmacology; Organ Transplantation; Swine; T-Lymphocytes, Cytotoxic - cytology; thymus; Thymus Gland - cytology; Thymus Gland - immunology; Thymus Gland - metabolism; Time Factors; tolerance; transplantation; Transplantation, Heterologous; Transplantation, Homologous
• ISSN: 1600-6135; 1600-6143
• DOI: 10.1046/j.1600-6143.2003.00295.x

We have generated a novel composite organ, the thymoheart, which facilitates the contemporaneous transfer of fully vascularized and functional donor thymic tissue to the host at the time of cardiac transplantation. Composite thymoheart allografts were prepared in MHC‐inbred miniature swine by implanting autologous thymic tissue into donor hearts 60–90 days before organ procurement. Thymoheart allografts and unmanipulated control hearts were then transplanted into three groups, each treated with the same 12 days of cyclosporine. MHC‐matched thymohearts transplanted into euthymic recipients had a minimum survival ranging between 72 and 194 days vs. 42–64 days for unmanipulated control hearts (p = 0.02). MHC class I‐disparate thymohearts transplanted into euthymic recipients had a minimum survival ranging between 64 and 191 days vs. 30–55 days for unmanipulated control hearts (p = 0.01). MHC class I‐disparate thymohearts transplanted into thymectomized recipients survived between 41 and 70 days vs. 8–27 days for unmanipulated control hearts (p = 0.01). Cellular and humoral functional assays, and skin grafting, confirmed the presence of donor‐specific hyporesponsiveness in long‐term thymoheart allografts recipients. The transfer of vascularized, functional donor thymic tissue to the host at the time of cardiac transplantation may provide a novel approach to the induction of tolerance in human heart transplant recipients.