Effects of short‐term severe and long‐term mild STZ‐induced diabetes in urethral tissue of female rats
Aims To estimate and compare the alterations in the urethral tissues of female rats with two diabetes models: short‐term severe and long‐term mild diabetes. Methods To induce mild diabetes (blood glucose levels between 120 and 300 mg/dl), female newborns received streptozotocin (100 mg/kg body weigh...
Saved in:
Published in | Neurourology and urodynamics Vol. 36; no. 3; pp. 574 - 579 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Wiley Subscription Services, Inc
01.03.2017
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Aims
To estimate and compare the alterations in the urethral tissues of female rats with two diabetes models: short‐term severe and long‐term mild diabetes.
Methods
To induce mild diabetes (blood glucose levels between 120 and 300 mg/dl), female newborns received streptozotocin (100 mg/kg body weight, sc route), and to induce short‐term severe diabetes (blood glucose levels > 300 mg/d), adult animals received streptozotocin (40 mg/kg, iv route). The rats were killed on day 133 of the experimental via an i.p. Thiopentax®injection of 80 mg/kg, and the urethrovaginal tissues were harvested. Morphometric, pathological, immunohistochemical, and ultrastructural analyses were conducted.
Results
In the long‐term mild diabetes group, collagen deposition, severe fibrosis, lipid droplets and numerous subsarcolemmal, and intermyofibrillar mitochondria were observed. In the short‐term severe diabetes group, centrally located myonuclei and a significantly reduced striated muscle area were noted. Both diabetic models exhibited similar immunohistochemistry patterns, with changes from fast to slow fibers and a decrease in the numbers of fast fibers.
Conclusions
Either long‐term mild hyperglycemia or short‐term severe hyperglycemia have detrimental impacts on muscle health. They are both involved in the failure to maintain healthy skeletal muscle that may contribute to the development of pelvic floor dysfunctions via different pathways. These results have important implications for monitoring and prevention strategies for improving the quality of life of women with diabetes mellitus and pelvic floor muscle dysfunction. Neurourol. Urodynam. 36:574–579, 2017. © 2016 Wiley Periodicals, Inc. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0733-2467 1520-6777 |
DOI: | 10.1002/nau.22974 |