Functional Ion Channels in Tethered Bilayer Membranes-Implications for Biosensors

• Published in: Chembiochem : a European journal of chemical biology Vol. 8; no. 11; pp. 1246 - 1250
• Main Authors: Keizer, Henk M., Dorvel, Brian R., Andersson, Martin, Fine, Daniel, Price, Rebecca B., Long, Joanna R., Dodabalapur, Ananth, Köper, Ingo, Knoll, Wolfgang, Anderson, Peter A. V., Duran, Randolph S.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 23.07.2007; WILEY‐VCH Verlag
• Subjects: Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Ion Channel Gating; ion channels; Ion Channels - metabolism; Lipid Bilayers - chemistry; lipids; membranes; Microarray Analysis; Molecular Structure; Probability; single-molecule studies

The ties that bind. A biosensor based on modulation of single ion‐channel activity, with the ability to detect analytes in the micromolar range was devised. Ion channels were interfaced to a gold surface and reconstituted into tethered bilayer lipid membranes (tBLMs; see scheme). Using this method w...