Functional Ion Channels in Tethered Bilayer Membranes-Implications for Biosensors

The ties that bind. A biosensor based on modulation of single ion‐channel activity, with the ability to detect analytes in the micromolar range was devised. Ion channels were interfaced to a gold surface and reconstituted into tethered bilayer lipid membranes (tBLMs; see scheme). Using this method w...

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Published inChembiochem : a European journal of chemical biology Vol. 8; no. 11; pp. 1246 - 1250
Main Authors Keizer, Henk M., Dorvel, Brian R., Andersson, Martin, Fine, Daniel, Price, Rebecca B., Long, Joanna R., Dodabalapur, Ananth, Köper, Ingo, Knoll, Wolfgang, Anderson, Peter A. V., Duran, Randolph S.
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 23.07.2007
WILEY‐VCH Verlag
Subjects
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Ion Channel Gating
ion channels
Ion Channels - metabolism
Lipid Bilayers - chemistry
lipids
membranes
Microarray Analysis
Molecular Structure
Probability
single-molecule studies
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Summary:The ties that bind. A biosensor based on modulation of single ion‐channel activity, with the ability to detect analytes in the micromolar range was devised. Ion channels were interfaced to a gold surface and reconstituted into tethered bilayer lipid membranes (tBLMs; see scheme). Using this method we obtained single channel activities from the synthetic ion channel, M2δ, and the Ca2+‐activated K+ ion channel.
Bibliography:DARPA MOLDICE program
NSF REU program
ArticleID:CBIC200700094
Knut and Alice Wallenbergs Foundation
ark:/67375/WNG-8Z5RRS8Z-8
AFOSR
istex:71CE4B982BF89A3E8EAFCBD62D84064B0F36DE00
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.200700094