Isolation and growth physiology of novel thermoactinomycetes

A total of 34 thermophilic isolates identified as members of the genus Thermoactinomyces by a range of chemotaxonomic, microscopic and determinative biochemical tests, were isolated from two acid soils. Growth studies in shake flask and fermenter identified the isolates to be moderately acidophilic...

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Bibliographic Details
Published inJournal of applied microbiology Vol. 83; no. 6; pp. 685 - 692
Main Authors Yallop, C.A, Edwards, C, Williams, S.T
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Science Ltd 01.12.1997
Blackwell Science
Subjects
acid soils
Actinomycetales - enzymology
Actinomycetales - growth & development
Actinomycetales - isolation & purification
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Isolation and description
Mission oriented research
soil bacteria
Temperature
Acidophily
Microorganism growth
Environmental factor
Thermoactinomyces
Characterization
Soils
Enzymatic activity
Morphology
Bacteria
Isolate
Isolation
Actinomycetes
Physiology
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Summary:A total of 34 thermophilic isolates identified as members of the genus Thermoactinomyces by a range of chemotaxonomic, microscopic and determinative biochemical tests, were isolated from two acid soils. Growth studies in shake flask and fermenter identified the isolates to be moderately acidophilic with growth occurring between pH 4.5 and 6.0 with optima at pH 5.0. The isolates differed considerably from known Thermoactinomyces cultures in their pH profile, colony morphology and in several biochemical tests. Extracellular enzyme activities are identified and partially characterized in terms of their thermostability, pH and temperature profiles from crude supernatant fluid samples. Optimal protease, amylase and pullulanase activity was observed at pH 5.0-5.5 and 75-80 degrees C with each showing T(50) values of 10, 30 and 30 min, respectively. A highly thermotolerant extracellular esterase was also identified which retained 50% activity after 8 h at 90 degrees C . This is the first report of an acidophilic member of the genus Thermoactinomyces.
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ISSN:1364-5072
1365-2672
DOI:10.1046/j.1365-2672.1997.00282.x