Regulation of ILT3 Gene Expression by Processing of Precursor Transcripts in Human Endothelial Cells
Immunoglobulin‐like transcript (ILT)‐3 is a transmembrane receptor, which belongs to the immunoglobulin superfamily. In previous studies, we showed that allospecific CD8+CD28− T suppressor cells (Ts) induce the expression of ILT3 in human endothelial cells (EC) rendering them tolerogenic. Using a po...
Saved in:
Published in | American journal of transplantation Vol. 6; no. 1; pp. 76 - 82 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford UK
Munksgaard International Publishers
01.01.2006
Blackwell |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Immunoglobulin‐like transcript (ILT)‐3 is a transmembrane receptor, which belongs to the immunoglobulin superfamily. In previous studies, we showed that allospecific CD8+CD28− T suppressor cells (Ts) induce the expression of ILT3 in human endothelial cells (EC) rendering them tolerogenic. Using a polymerase chain reaction (PCR)‐based approach, we now demonstrate by cell fractionation and sequencing studies that ILT3 precursor RNA is expressed and retained in nuclei of resting EC. Ts interaction with EC or exposure of EC to interleukin‐10 (IL‐10) and interferon alpha (IFN‐α) triggers processing of ILT3 pre‐mRNA. Western blot analysis showed that the expression of the mature ILT3 transcript is accompanied by production of ILT3 protein. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1600-6135 1600-6143 |
DOI: | 10.1111/j.1600-6143.2005.01162.x |