Regulation of ILT3 Gene Expression by Processing of Precursor Transcripts in Human Endothelial Cells

• Published in: American journal of transplantation Vol. 6; no. 1; pp. 76 - 82
• Main Authors: Kim‐Schulze, S., Seki, T., Vlad, G., Scotto, L., Fan, J., Colombo, P. C., Liu, J., Cortesini, R., Suciu‐Foca, N.
• Format: Journal Article
• Language: English
• Published: Oxford UK Munksgaard International Publishers 01.01.2006; Blackwell
• Subjects: Activation‐induced splicing; Biological and medical sciences; CD28 Antigens - analysis; CD8-Positive T-Lymphocytes - immunology; Cell Fractionation; Cell Nucleus - chemistry; Cell Nucleus - metabolism; Endothelium, Vascular - chemistry; Endothelium, Vascular - immunology; Endothelium, Vascular - metabolism; Gene Expression Regulation; Humans; immunoglobulin‐like transcript 3; Medical sciences; Membrane Glycoproteins; Receptors, Cell Surface - analysis; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; Receptors, Immunologic; RNA Precursors - metabolism; RNA Processing, Post-Transcriptional; RNA, Messenger - analysis; RNA, Messenger - metabolism; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; T-Lymphocytes - immunology; tolerogenic endothelial cells; Transcription, Genetic; Human; Endothelial cell; Splicing; Precursor cell; Activation; Gene expression; Inhibitory Receptor; Regulation(control); Messenger RNA; tolerogenic endothelial cells; Gene; Immune tolerance; immunoglobulin-like transcript 3; ILT3 receptor; Activation-induced splicing
• ISSN: 1600-6135; 1600-6143
• DOI: 10.1111/j.1600-6143.2005.01162.x

Immunoglobulin‐like transcript (ILT)‐3 is a transmembrane receptor, which belongs to the immunoglobulin superfamily. In previous studies, we showed that allospecific CD8+CD28− T suppressor cells (Ts) induce the expression of ILT3 in human endothelial cells (EC) rendering them tolerogenic. Using a polymerase chain reaction (PCR)‐based approach, we now demonstrate by cell fractionation and sequencing studies that ILT3 precursor RNA is expressed and retained in nuclei of resting EC. Ts interaction with EC or exposure of EC to interleukin‐10 (IL‐10) and interferon alpha (IFN‐α) triggers processing of ILT3 pre‐mRNA. Western blot analysis showed that the expression of the mature ILT3 transcript is accompanied by production of ILT3 protein.