Does benign prostatic hyperplasia originate from the peripheral zone of the prostate? A preliminary study

• Published in: BJU international Vol. 100; no. 5; pp. 1091 - 1096
• Main Authors: Tang, Jie, Yang, Jing Chun, Zhang, Yan, Liu, Xin, Zhang, Lihai, Wang, Zhili, Li, Junlai, Luo, Yukun, Xu, Jianhong, Shi, Huaiyin
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.11.2007; Blackwell
• Subjects: Aged; Aged, 80 and over; benign prostatic hyperplasia; Biological and medical sciences; Cell Proliferation; Gynecology. Andrology. Obstetrics; Humans; hypoechoic nodule; Immunohistochemistry; Male; Male genital diseases; Medical sciences; Middle Aged; Nephrology. Urinary tract diseases; peripheral zone; Prostate - diagnostic imaging; Prostate - metabolism; Prostate - pathology; Prostatic Hyperplasia - diagnostic imaging; Prostatic Hyperplasia - metabolism; Prostatic Hyperplasia - pathology; Proto-Oncogene Proteins c-bcl-2 - metabolism; Receptor, Epidermal Growth Factor - metabolism; Receptors, Androgen - metabolism; Transforming Growth Factor beta1 - metabolism; transrectal ultrasonography; Tumors; Tumors of the urinary system; Ultrasonography; Urinary tract. Prostate gland; Sonography; Human; Nephrology; Urinary system disease; Prostate disease; Nodule; Urology; Periphery; Anatomic pathology; Histopathology; benign prostatic hyperplasia; peripheral zone; hypoechoic nodule; Echography; Benign prostatic hyperplasia; Benign neoplasm; transrectal ultrasonography; Male genital diseases
• ISSN: 1464-4096; 1464-410X
• DOI: 10.1111/j.1464-410X.2007.07081.x

OBJECTIVE To compare the histological characteristics, cell proliferation, apoptosis and biological features in benign prostatic hyperplasia (BPH) in the peripheral (PZ) and transition zone (TZ) of the prostate. PATIENTS AND METHODS Tissue from BPH in TZ and PZ was obtained from 68 patients undergoing transrectal ultrasonography‐guided biopsy and used for both morphometric analysis and immunohistochemical studies. The epithelial, stromal and luminal composition of the tissue was determined using a computer‐assisted method for quantitative morphometric analysis. Apoptosis was detected as the apoptotic index (AI) using the TdT dUTP nick‐end labelling assay. Cell proliferation was determined as the proliferation index (PI) using Ki‐67 immunostaining. The expression of epidermal growth factor receptor (EGFR), transforming growth factor β1 (TGFβ1), androgen receptor (AR) and bcl‐2 were assessed immunohistochemically. RESULTS There was no difference in the stroma/epithelium ratio between PZ and TZ hyperplastic nodules (P > 0.05). The mean AI in epithelium was almost identical to the corresponding PI. In stroma, no apoptotic cells were detectable. There was a significantly higher PI and AI in the glandular epithelial cells in PZ hyperplastic than in TZ hyperplastic nodules, but no difference in PI of the stromal cells between PZ and TZ hyperplastic nodules. There was significantly higher expression of TGFβ1 and lower expression of EGFR and bcl‐2 in PZ than TZ hyperplastic nodules (P < 0.05). There was no difference in AR expression between PZ and TZ hyperplastic nodules (P > 0.05). CONCLUSIONS These results indicate that some hyperplastic nodules in PZ might originate from the PZ, and the formation of these nodules might be modulated in a different way from that in the TZ.