Cross-Linking of Transmembrane Helices Reveals a Rigid-Body Mechanism in Bacteriorhodopsin Transport
A flashy protein: The flash‐induced transient protonation of pyranine has been studied in the presence of the cross‐linked double mutant E166C/A228C (red trace), a reduced mutant (blue trace), and wild‐type bacteriorhodopsin (gray traces). If helices F and G are cross‐linked, there is a delay in bot...
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Published in | Angewandte Chemie (International ed.) Vol. 48; no. 45; pp. 8523 - 8525 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
WILEY-VCH Verlag
26.10.2009
WILEY‐VCH Verlag |
Subjects | |
Online Access | Get full text |
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Summary: | A flashy protein: The flash‐induced transient protonation of pyranine has been studied in the presence of the cross‐linked double mutant E166C/A228C (red trace), a reduced mutant (blue trace), and wild‐type bacteriorhodopsin (gray traces). If helices F and G are cross‐linked, there is a delay in both proton release (extracellular side) and proton uptake (cytoplasmic side). Together with flash photolysis and FTIR studies, these data support a rigid‐body mechanism of bacteriorhodopsin proton transport. |
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Bibliography: | ark:/67375/WNG-C2ZRRNT2-6 ArticleID:ANIE200904031 We thank Dr. Víctor Lórenz-Fonfría and Dr. Pere Garriga for helpful discussions, Dr. Enric Querol for advice at the beginning of this work, Jordi Hernando for help in the flash photolysis experiments, Elodia Serrano and Neus Ontiveros for excellent technical assistance, and Dr. Mathias Lübben (Ruhr-Universität Bochum) for providing the pEF191 vector and the MPK409 strain. This work was supported by a Direcció General de Recerca fellowship (2007PIV10038) to T.L. and by a Dirección General de Investigación grant (BFU2006-04656/BMC). istex:CEE40F9F81FA463BC656F80189380736041BFF3B Dirección General de Investigación - No. BFU2006-04656/BMC We thank Dr. Víctor Lórenz‐Fonfría and Dr. Pere Garriga for helpful discussions, Dr. Enric Querol for advice at the beginning of this work, Jordi Hernando for help in the flash photolysis experiments, Elodia Serrano and Neus Ontiveros for excellent technical assistance, and Dr. Mathias Lübben (Ruhr‐Universität Bochum) for providing the pEF191 vector and the MPK409 strain. This work was supported by a Direcció General de Recerca fellowship (2007PIV10038) to T.L. and by a Dirección General de Investigación grant (BFU2006‐04656/BMC). ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1433-7851 1521-3773 |
DOI: | 10.1002/anie.200904031 |