Monoclonal antibody against species-specific epitope of Pseudomonas aeruginosa Hsp60 protein cross-reacts with Pseudomonas stutzeri and other Pseudomonas species

• Published in: FEMS microbiology letters Vol. 154; no. 1; pp. 131 - 137
• Main Authors: Lüneberg, Edeltraud, Müller, Dirk, Steinmetz, Ivo, Frosch, Matthias
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.09.1997; Oxford University Press
• Subjects: Alcaligenes; Amino Acid Sequence; Amino acids; Antibodies, Monoclonal; Antibody Specificity; Bacteria; Bacterial Proteins - analysis; Bacterial Proteins - genetics; Bacterial Proteins - immunology; Blotting, Western; Chaperonin 60 - analysis; Chaperonin 60 - genetics; Chaperonin 60 - immunology; Cloning, Molecular; Cross Reactions; Cross-reaction; Cross-reactivity; Deoxyribonucleic acid; DNA; DNA, Bacterial - analysis; E coli; Epitopes; Epitopes - analysis; Epitopes - immunology; Heat shock protein; Heat shock proteins; Homology; HSP60 gene; Hsp60 protein; Microbiology; Molecular cloning; Molecular Sequence Data; Molecular structure; Monoclonal antibodies; Nucleotide sequence; Nucleotides; Phylogeny; Proteins; Pseudomonas; Pseudomonas aeruginosa; Pseudomonas aeruginosa - chemistry; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - immunology; rRNA; Sequence Analysis, DNA; Species; Species Specificity; Molecular cloning; gene; Heat shock protein
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.1997.tb12634.x

Abstract In a previous study, we determined the epitope of the Pseudomonas aeruginosa Hsp60 heat shock protein which is recognized by the specific monoclonal antibody (MAb) 2528. Subsequent investigations revealed a weak cross-reactivity of MAb 2528 with P. stutzeri, P. alcaligenes, P. mendocina and P. pseudoalcaligenes. To elucidate the molecular structure for these cross-reactions, we cloned the P. stutzeri hsp60 gene in Escherichia coli and determined the nucleotide sequence of the gene. In addition, the hsp60 gene of further Pseudomonas species was amplified and sequenced and amino acid substitutions within the epitope recognized by MAb 2528 were determined. The decapeptide QADIEARVLQ is unique to the P. aeruginosa Hsp60 protein, and cross-reaction of MAb 2528 reflects the phylogenetic relationship of Pseudomonas species as P. aeruginosa and all four cross-reacting species constitute a DNA homology group within the rRNA group I of the family Pseudomonadaceae, which belong to the γ- subclass of the Proteobacteria.