Exploring a natural MDR reversal agent: potential of medicinal food supplement Nerium oleander leaf distillate

• Published in: Asian Pacific journal of tropical biomedicine Vol. 3; no. 8; pp. 644 - 649
• Main Author: Meltem Demirel Kars Ufuk Gündüz Kamilüney AhmetLevent Ba
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.08.2013; Asian Pacific Tropical Medicine Press
• Subjects: activity; Antineoplastic Agents, Phytogenic - pharmacology; ATP Binding Cassette Transporter, Sub-Family B; ATP-Binding Cassette, Sub-Family B, Member 1 - genetics; ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism; Basic Researches; Biphenyl Compounds - metabolism; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Dietary Supplements - analysis; Drug Resistance, Multiple - drug effects; Female; Flow Cytometry; food; FRSA; FRSA activity; Gene Expression Regulation, Neoplastic; Humans; MCF-7; MCF-7 Cells - drug effects; MCF-7 Cells - metabolism; MDR; MDR reversal; Medicinal; Medicinal food supplement; Nerium; Nerium - chemistry; Nerium oleander; oleander; Paclitaxel - pharmacology; Picrates - metabolism; Plant Extracts - chemistry; Plant Leaves - chemistry; Polymerase Chain Reaction; reversal; supplement; Vincristine - pharmacology; Medicinal food supplement; MCF–7; FRSA activity; MDR reversal; Nerium oleander; MCF-7
• ISSN: 2221-1691; 2588-9222
• DOI: 10.1016/S2221-1691(13)60130-6

Objective:To investigate the molecular effects ofNerium oleanderleaf distillate on paclitaxel and vincristine resistant(MCF-7/Pac and MCF-7/Vinc)cells and sensitive(MCF-7/S)cell lines.Methods:Nerium oleander(N.oleander)leaf extract was obtained by hydrodistillation method.The toxicological effects ofN.oleanderdistillate,previously suggested as medicinal food supplement,on drug resistant cells were evaluated by XTT tests.MDR modulation potential of the plant material was evaluated by flow cytometry and fluorescent microscopy.Paclitaxel and vincristine were applied to the sublines in combination with N.oleanderdistillate.Results:Fractional inhibitory indices show thatN.oleanderdistillate did not increase the antiproliferative effects of anticancer drugs.N.oleandertreatment in to MCF-7/Pac and MCF-7/Vinc did not inhibit P-gp activity and MDR1 gene expression level.Conclusions:As a result it may be suggested that althoughN.oleanderdistillate has some medicinal effects as food supplement it may not be suitable as an MDR modulator for drug resistant breast cancer cells.