Chiral separation of halogenated amino acids by ligand-exchange capillary electrophoresis

• Published in: Electrophoresis Vol. 26; no. 20; pp. 3878 - 3883
• Main Authors: Koidl, Julia, Hödl, Heike, Schmid, Martin G., Pantcheva, Svetlana, Pajpanova, Tamara, Gübitz, Gerald
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.10.2005; WILEY‐VCH Verlag
• Subjects: Amino Acids - isolation & purification; Capillary electrophoresis; Copper; Electrophoresis, Capillary - methods; Electrophoresis, Capillary - standards; Enantiomer separation; Halogenated amino acids; Halogens; Histidine - isolation & purification; Hydroxyproline - isolation & purification; Ligand-exchange; Ligands; Stereoisomerism
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/elps.200500130

The chiral separation of halogenated amino acids by ligand‐exchange CE is described. Halogenated amino acids attracted increasing interest in recent years because of their physiological activities. Different chiral selectors, as there are L‐4‐hydroxyproline, L‐histidine, and N‐alkyl derivatives of L‐4‐hydroxyproline in form of their copper(II) complexes, are compared for their chiral recognition ability for halogenated amino acids. The influence of various parameters, such as selector concentration, pH, organic modifier, and field strength, on the resolution was investigated. All halogenated amino acids investigated were baseline‐separated under optimized conditions.