Role of trehalose dimycolate-induced interferon-α/β in the restriction of encephalomyocarditis virus growth in vivo and in peritoneal macrophage cultures

• Published in: Antiviral research Vol. 28; no. 2; pp. 175 - 189
• Main Authors: Guillemard, E., Geniteau-Legendre, M., Kergot, R., Lemaire, G., Petit, J.F., Labarre, C., Quero, A.M.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.10.1995; Elsevier
• Subjects: Animals; Antiviral Agents - pharmacology; Ascitic Fluid - immunology; Biological and medical sciences; Cardiovirus Infections - drug therapy; Cardiovirus Infections - immunology; Cell Line; Cells, Cultured; Cord Factors - pharmacology; Disease Models, Animal; Encephalomyocarditis (EMC) virus; encephalomyocarditis virus; Encephalomyocarditis virus - drug effects; Encephalomyocarditis virus - immunology; Female; Immunomodulators; Interferon-alpha - biosynthesis; Interferon-beta - biosynthesis; Interferon-α/β; Macrophage; Macrophages, Peritoneal - virology; Medical sciences; Mice; Pharmacology. Drug treatments; Trehalose dimycolate (TDM); Interferon-α/β; Trehalose dimycolate (TDM); Encephalomyocarditis (EMC) virus; Macrophage; Cell culture; Intraperitoneal administration; Alpha interferon; Cytokine; Rodentia; In vitro; Prevention; Virus; In vivo; Vertebrata; Chemotherapy; Mammalia; Mouse; Animal; Beta interferon; Encephalomyocarditis
• ISSN: 0166-3542; 1872-9096
• DOI: 10.1016/0166-3542(95)00047-P

Preventive intraperitoneal trehalose dimycolate (TDM) treatment of mice, inoculated with encephalomyocarditis (EMC) virus by the same route, caused restriction of virus growth in the peritoneum, which was correlated to IFN production in peritoneal fluids prior to infection. Peritoneal macrophages from TDM-treated mice (TDM-PM) spontaneously secreted IFN-α/β in large amounts. By their supernatants, TDM-PM could transfer an antiviral state against EMC virus to permissive resident peritoneal macrophages from control mice. IFN-α/β produced by TDM-PM was found to be involved in this transfer activity. TDM-PM also exerted a strong antiviral effect on EMC virus-infected L-929 cells, which increased with time and the macrophage-target cell ratio. This activity also occurred by an IFN-α/β-dependent mechanism. These data point to the role of IFN-α/β production prior to EMC virus infection in the antiviral activities of TDM-PM and, more generally, in the outcome of viral infection.