Biomarkers of meat tenderness: Present knowledge and perspectives in regards to our current understanding of the mechanisms involved

• Published in: Meat science Vol. 95; no. 4; pp. 854 - 870
• Main Authors: Ouali, Ahmed, Gagaoua, Mohammed, Boudida, Yasmine, Becila, Samira, Boudjellal, Abdelghani, Herrera-Mendez, Carlos H., Sentandreu, Miguel A.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2013; Elsevier
• Subjects: Animals; Annexins - metabolism; Apoptosis; Apoptosis - physiology; Biomarkers; Caspases - metabolism; Chemical and Process Engineering; Energy Metabolism; Engineering Sciences; Food engineering; Food Quality; Food Technology; Galectin 1 - metabolism; Glycolysis - physiology; Heat-Shock Proteins - metabolism; Life Sciences; Meat - analysis; Meat quality; Metabolism; Mitochondria - metabolism; Muscle cell; Muscle, Skeletal - chemistry; Oxidation-Reduction; Peptide Hydrolases - metabolism; Peroxiredoxin VI - metabolism; Postmortem Changes; Protease Inhibitors - metabolism; Proteolysis; Tenderness; Biomarkers; Muscle cell; Metabolism; Tenderness; Meat quality; Apoptosis; LONGISSIMUS-THORACIS MUSCLE; INDUCED APOPTOSIS; 2-DIMENSIONAL GEL-ELECTROPHORESIS; CELL-DEATH; MITOCHONDRIAL FISSION; BOVINE SKELETAL-MUSCLE; HEAT-SHOCK PROTEINS; AMINO-ACID-METABOLISM; INTRAMUSCULAR CONNECTIVE-TISSUE; MEMBRANE PERMEABILIZATION
• ISSN: 0309-1740; 1873-4138
• DOI: 10.1016/j.meatsci.2013.05.010

Biomarkers of the meat quality are of prime importance for meat industry, which has to satisfy consumers' expectations and, for them, meat tenderness is and will remain the primary and most important quality attribute. The tenderization of meat starts immediately after animal death with the onset of apoptosis followed by a cooperative action of endogenous proteolytic systems. Before consideration of the biomarkers identified so far, we present here some new features on the apoptotic process. Among them, the most important is the recent discovery of a complex family of serpins capable to inhibit, in a pseudo-irreversible manner, caspases, the major enzymes responsible of cell dismantling during apoptosis. The biomarkers so far identified have been then sorted and grouped according to their common biological functions. All of them refer to a series of biological pathways including glycolytic and oxidative energy production, cell detoxification, protease inhibition and production of Heat Shock Proteins. Some unusual biomarkers are also presented: annexins, galectins and peroxiredoxins. On this basis, a detailed analysis of these metabolic pathways allowed us to identify some domains of interest for future investigations. It was thus emphasized that mitochondria, an important organelle in the production of energy from carbohydrates, lipids and proteins are a central element in the initiation and development of apoptosis. It was therefore stressed forward that, in fact, very little is known about the postmortem fate of these organelles and their multiple associated activities. Other topics discussed here would provide avenues for the future in the context of identifying reliable predictors of the ultimate meat tenderness. •New features in postmortem muscle: phagocytosis and caspase/apoptosis inhibitors•Biomarkers of meat tenderness identified and their related metabolic pathways•The different source of energy used in postmortem muscle: amino acid, fat and sugar•Mitochondria: a key control point in energy production and apoptosis regulation•Inhibitors of caspases/apoptosis and major role of inhibitors in meat proteolysis