Immune sera to individual Borrelia burgdorferi isolates or recombinant OspA thereof protect SCID mice against infection with homologous strains but only partially or not at all against those of different OspA/OspB genotype

• Published in: Vaccine Vol. 11; no. 10; pp. 1049 - 1054
• Main Authors: Schaible, Ulrich E., Wallich, Rainer, Kramer, Michael D., Gern, Lise, Anderson, John F., Museteanu, C., Simon, Markus M.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 1993; Elsevier
• Subjects: Animals; Antigens, Bacterial; Antigens, Surface - genetics; Antigens, Surface - immunology; Bacterial Outer Membrane Proteins - genetics; Bacterial Outer Membrane Proteins - immunology; Bacterial Vaccines; Bacteriology; Biological and medical sciences; Borrelia burgdorferi; Borrelia burgdorferi Group - immunology; cross-protection; Female; Fundamental and applied biological sciences. Psychology; Genotype; heterogeneity; Immune Sera - immunology; immunity; Lipoproteins; Lyme disease; Lyme Disease - prevention & control; Male; Mice; Mice, Inbred C57BL; Mice, Inbred DBA; Mice, SCID; Microbiology; outer surface proteins; Recombinant Proteins - immunology; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Lyme disease; outer surface proteins; Borrelia burgdorferi; immunity; heterogeneity; cross-protection; Immunoprotection; Animal model; Membrane protein; Immune response; Spirochaetaceae; Spirochaetales; Rodentia; Genotype; Passive immunity; Cell surface; Vertebrata; Mammalia; Antiserum; Mouse; Bacteria; Isolate; Severe; Combined immune deficiency
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/0264-410X(93)90132-H

The outer surface proteins OspA and OspB of Borrelia burgdorferi have recently been demonstrated to be major target proteins for protective antibodies in mice against infection with the homologous spirochaetal strain. However, it has become clear from a variety of studies that B. burgdorferi isolates of different geographical origin and/or sources are heterogenous and that they can be divided into at least six subgroups according to their distinct OpsA/OspB genotypes. In order to analyse cross-protection between these subgroups we have now generated immune sera to various isolates of B. burgdorferi with different OspA/OspB genotypes. We show that passive immunization with antisera specific for whole spirochaetes or recombinant OspA of one spirochaetal isolate protects severe combined immunodeficiency mice against infection with strains of the corresponding OspA/OspB genotype but only partially or not at all against infection with isolates expressing distinct OspA/OspB genotypes. The incomplete protection mediated by individual antisera against independent isolates of B. burgdorferi suggests that an effective subunit vaccine against Lyme disease should consist of a mixture of OspA structures covering the heterogeneity of this protein within the species B. burgdorferi.