Biodiversity of South Indian tea clones with detection of plant-based adulterants in tea dust using DNA barcoding

• Published in: Natural product research Vol. 36; no. 18; pp. 4608 - 4613
• Main Authors: Avarave, Steffi, Thomas, Jibu
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 01.09.2022; Taylor & Francis Ltd
• Subjects: Adulterants; adulteration; Algorithms; Biodiversity; Chloroplasts; Cloning; commercial tea products; Cultivars; Deoxyribonucleic acid; DNA; DNA barcoding; Dust; Gene polymorphism; Gene sequencing; Genetic analysis; Markers; Phylogeny; RpoB protein; South Indian tea cultivars; TA cloning; Tea
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2021.2002325

Tea is by and large a highly penetrated product in south India. Hence the adulteration risk in tea dust gets hiked in the markets. We constructed a standard database using plant plastid markers (rbcL, matK, trnH-psbA, rpoC, rpoB, ycf 1) and nuclear (ITS2) locus from prominent south Indian tea clones representing Assam, China, and Cambod varieties. These barcodes were used as reference algorithm to investigate the authenticity of 10 sampled commercial tea dust by recovering its DNA barcodes using rbcL, matK, and ITS2 loci. PCR amplification success, sequencing efficiency, genetic polymorphisms, BLAST search, and phylogenetic analysis were performed to enhance genotypic information on south tea cultivars and in authenticating the commercial samples of Camellia sinensis. Findings suggest that the chloroplast and nuclear loci can identify tea plant at the genus and varietal level respectively and rbcL as the potential marker for detecting plant-based admixtures coupled with TA cloning after DNA barcoding.