Presynaptic NK1 Receptor Activation by Substance P Suppresses EPSCs via Nitric Oxide Synthesis in the Rat Insular Cortex

• Published in: Neuroscience Vol. 455; pp. 151 - 164
• Main Authors: Matsumura, Sachie, Yamamoto, Kiyofumi, Nakaya, Yuka, O'Hashi, Kazunori, Kaneko, Keisuke, Takei, Hiroki, Tsuda, Hiromasa, Shirakawa, Tetsuo, Kobayashi, Masayuki
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 10.02.2021
• Subjects: Animals; Cerebral Cortex; Excitatory Postsynaptic Potentials; glutamate receptor; Nitric Oxide; nociception; pyramidal cell; Rats; Receptors, Presynaptic; Substance P; Synaptic Transmission; tachykinin; NO; PV; EPSCs; nociception; ACSF; VGAT; tachykinin; cerebral cortex; PPR; PNs; Sar-Met; NK1; NONOate; NPY; glutamate receptor; nNOS; eEPSCs; pyramidal cell; mEPSCs; IC; SEM; DAX-J2; SP
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2020.12.012

•Substance P suppressed EPSCs recorded from pyramidal cells via NK1 receptors.•Substance P increased the paired-pulse ratio and failure rate.•Substance P synthesized NO principally in a subset of GABAergic neurons.•Substance P-induced EPSC suppression was blocked by an NOS inhibitor/NO scavenger.•NO synthesized by substance P may reduce release probability of glutamate. Substance P (SP) regulates inhibitory synaptic transmission mediated by GABAA receptors in the cerebral cortex; however, SP-mediated regulation of excitatory synaptic transmission remains poorly understood. We performed whole-cell patch-clamp recordings from pyramidal neurons to examine the effects of SP on excitatory postsynaptic currents (EPSCs) mediated via AMPA receptors in the insular cortex (IC), which is involved in nociceptive information processing. First, EPSCs evoked by minimal electrical stimulation (eEPSCs) including stepwise EPSCs and failure events, were examined. SP dose-dependently suppressed mean eEPSC amplitude, partially due to an increase in the failure rate of eEPSCs. The SP-induced suppression of eEPSCs was accompanied by an increase in the paired-pulse ratio and was inhibited by the preapplication of SR140333, an NK1 receptor antagonist. [Sar9,Met(O2)11]-substance P, an NK1 receptor-selective agonist, mimicked the effects of SP on eEPSCs and decreased the frequency of miniature EPSCs (mEPSCs) without changing the average mEPSC amplitude. Considering that most NK1 receptors in the cerebral cortex are expressed in nitric oxide synthase (NOS)-positive GABAergic neurons, the SP-induced suppressive effect on EPSCs may be mediated by nitric oxide (NO) in this subtype of GABAergic neurons. NO imaging using the fluorescent probe DAX-J2 Red supports this hypothesis: SP increased the fluorescence intensity of DAX-J2 Red in some GABAergic neurons. Furthermore, both L-NAME, an NOS inhibitor, and PTIO, an NO scavenger, diminished the SP-induced suppression of eEPSCs. These results suggest that the activation of presynaptic NK1 receptors contributes to SP-induced eEPSC suppression by activating the NO synthesis pathway in GABAergic neurons. (246 words)